Each bottle contains 100 grams of powder, which is sufficient to prepare 1 liter of 10X MESA (MOPS-EDTA sodium acetate) buffer.
M5755
MOPS-EDTA-Sodium Acetate (MESA)
powder for RNA electrophoresis buffers
Synonym(s):
MESA Buffer
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| Size/SKU | Availability | Price |
|---|---|---|
1 L | Please contact Customer Service for Availability | 7 550,00 CZK |
About This Item
NACRES:
NA.25
UNSPSC Code:
12161700
grade
Molecular Biology
Quality Segment
product line
BioReagent
form
powder
technique(s)
electrophoresis: suitable, nucleic acid detection: suitable
pH
6.8-7.2(reconstituted per directions)
solubility
H2O: soluble, faintly yellow
foreign activity
DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected
storage temp.
room temp
General description
MOPS – EDTA – Sodium Acetate (MESA) is the most commonly used running buffer for RNA electrophoresis prior to Northern blotting procedures. Applied voltage of 5 V/cm is recommended for maximum resolution.
Application
Suitable for use when making formaldehyde-agarose gels and associated running buffer for RNA electrophoresis.
Preparation Note
1x MESA buffer contains:
- 40 mM MOPS
- 10 mM sodium acetate
- 1 mM EDTA (pH 8.3)
Dissolve entire contents of bottle in a final volume of 1L molecular biology grade water. This produces a 10x MESA stock solution that can be further diluted as needed.
1 of 1
This Item | |||
|---|---|---|---|
| description powder for RNA electrophoresis buffers | description for Northern and Southern blotting, solution | description BioUltra, Molecular Biology, pH 7.4 | description BioUltra, Molecular Biology, ≥99.5% (titration) |
| technique(s) electrophoresis: suitable | technique(s) - | technique(s) - | technique(s) - |
| grade Molecular Biology | grade Molecular Biology | grade Molecular Biology | grade Molecular Biology |
| product line BioReagent | product line BioReagent | product line BioUltra | product line BioUltra |
| Quality Level 200 | Quality Level 100 | Quality Level 100 | Quality Level 200 |
| form powder | form solution | form liquid | form powder or crystals |
| pH 6.8-7.2(reconstituted per directions) | pH - | pH 7.4±0.2 (25 °C) | pH 2.5-4.0 (25 °C, 1 M in H2O) |
Storage Class
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Gloves, type N95 (US)
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