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W1754

Water

PCR Reagent, suitable for PCR

Synonym(s):

H2O

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1 vial
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CZK 938.00
5 vials
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CZK 3,250.00

About This Item

Linear Formula:
H2O
CAS Number:
Molecular Weight:
18.02
NACRES:
NA.25
PubChem Substance ID:
UNSPSC Code:
12191602
EC Number:
231-791-2
MDL number:
Beilstein/REAXYS Number:
2050024
Technique(s):
PCR: suitable
Bp:
100 °C (lit.)
Vapor pressure:
3 mmHg

CZK 938.00


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Product Name

Water, PCR Reagent

vapor density

<1 (vs air)

Quality Level

vapor pressure

3 mmHg

sterility

sterile-filtered

form

liquid

packaging

vial of 1.5 mL

technique(s)

PCR: suitable

refractive index

n20/D 1.34 (lit.)

pH

5-7

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1 of 1

This Item
952849801-OP4.86505
technique(s)

PCR: suitable

technique(s)

FISH: suitable

technique(s)

-

technique(s)

-

Quality Level

200

Quality Level

100

Quality Level

100

Quality Level

300

form

liquid

form

liquid

form

liquid

form

liquid

pH

5-7

pH

5-7

pH

-

pH

-

bp

100 °C (lit.)

bp

100 °C (lit.)

bp

100 °C (lit.)

bp

100 °C (lit.)

vapor density

<1 (vs air)

vapor density

-

vapor density

-

vapor density

-

General description

PCR grade water is sterile-filtered. It is free from exonucleases (DNAse, RNAse) and endonuclease (NICKase) and is also free from nucleic acid contamination.

Application

Suitable for polymerase chain reaction (PCR)
Water has been used:

  • as a component of the reaction mixture and as a diluting agent in microfluidic RT-qPCR
  • as a component of the reaction mixture for the amplification of products from fungal (Trametes versicolor) DNA
  • as a diluting agent and as a component of the reaction mixture for the amplification of cDNA[1]
  • Water has been used to make up the final volume of the sample in polymerase chain reaction (PCR)[1]

Other Notes

Easily compare specifications for Water products with the Water specification table.

Storage Class

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves


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Francine Marciano-Cabral et al.
Applied and environmental microbiology, 69(10), 5864-5869 (2003-10-09)
The free-living amoeboflagellate Naegleria fowleri is the causative agent of primary amoebic meningoencephalitis (PAM), a rapidly fatal disease of the central nervous system. In the United States, the disease is generally acquired while swimming and diving in freshwater lakes and
Multiplex PCR for rapid detection of genes encoding acquired metallo-beta-lactamases.
Matthew J Ellington et al.
The Journal of antimicrobial chemotherapy, 59(2), 321-322 (2006-12-23)
Incidence and survival of non-O157 verocytotoxigenic Escherichia coli in soil
Bolton DJ, et al.
Journal of Applied Microbiology, 111(2), 484-490 (2011)
If you?ve seen one worm, have you seen them all? Spatial, community, and genetic variability of tubificid communities in Montana
Lodh N, et al.
Freshwater science, 34(3), 909-917 (2015)
N Wellinghausen et al.
Applied and environmental microbiology, 67(9), 3985-3993 (2001-08-30)
Contamination of hospital water systems with legionellae is a well-known cause of nosocomial legionellosis. We describe a new real-time LightCycler PCR assay for quantitative determination of legionellae in potable water samples. Primers that amplify both a 386-bp fragment of the

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Protocols

Náš protokol SYBR Green qPCR je metoda určená k přesné kvantifikaci genové exprese a reakcí RT-PCR.

Naučte se standardní kroky protokolu PCR a zkontrolujte seznamy činidel nebo parametry cyklování. Tato metoda pro rutinní amplifikaci DNA pomocí PCR používá standardní Taq DNA polymerázu.

Reverzní transkripce analyzuje genovou expresi mRNA a čelí přitom problémům, jako jsou rozdíly v poločasu rozpadu transkriptů a časové vzorce.

Optimalizace podmínek qPCR je důležitá pro vývoj robustního testu. Dva hlavní přístupy jsou optimalizace koncentrace primerů a/nebo teploty žíhání.

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Global Trade Item Number

SKUGTIN
W1754-5VL04061837488696
W1754-1VL04061837488689

Questions

  1. 容量がわかりません。型番の1 VLとは1000 mLのことですか?

    1 answer
    1. As mentioned in the 'PROPERTIES' section, the volume of each vial is 1.5 mL.

      Helpful?

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