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Examination of Efficacy Specificity and Efficiency of an shRNA Lentiviral Library
LS Quarterly
Examination of Efficacy,
Specificity, and
Efficiency of an shRNA
Lentiviral Library
Betsy Boedeker,* Gregory A. Wemhoff, Andrea Spencer,
Roger Chiu, Suzanne Miller, Henry George, and
Edward J. Weinstein
*Corresponding Author: Betsy Boedeker, Functional Genomics,
Sigma-Aldrich, St. Louis, Missouri, USA
E-mail:
Checklist- EC400
The life science business of Merck KGaA, Darmstadt, Germany
operates as MilliporeSigma in the U.S. and Canada.
© 2022 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.
20448843w-CL Rev 04/22
Punch a hole in
Development of a chemiluminescent immunoassay (CLIA) using Estapor®magnetic microspheres
Application Note
Development of a chemiluminescent
immunoassay (CLIA) using
Estapor® magnetic microspheres
Introduction
Many factors must be considered before the
development of an immunoassay. One critical
consideration is ‘sensitivity’. What level of analyte
do you need to detect?
Product Information Sheet - PPB012
PPB012 - Product Information Sheet
Product Information Sheet
1% Agarose in Tris-Acetate-EDTA
User Guide
Art. 1.10537.0001 Carrez-Klärung (5fach-Konzentrat)
Reagenziensatz für die Probenvorbereitung in der
Lebensmittelanalytik
Packungsinhalt, Lagerung und Stabilität
Carrez-Lösung I: enthält Kaliumhexacyanoferrat(II)-Trihydrat (K4[Fe(CN)6] x 3H2O)
100 ml Konzentration: 15 g/100 ml
Carrez-Lösung II: enthält Zinksulfat-Heptahydrat
Data Sheet - SRP5070
PRKX, active (SRp5070) - Datasheet
PRKX, active, GST tagged, human
PRECISIO Kinase
recombinant, expressed in Sf9 cells
Catalog Number SRP5070
Storage Temperature –70°C
Synonym: PKX1
Product Description
PRKX is a serine/threonine protein kinase that
ERalpha Hu-Cy3 SmartFlare™ RNA Detection Probe
Background
SPECIES LEGEND: Hu Human Ms Mouse Rt Rat Rb Rabbit WR Most Common Vertebrates ( ) Predicted Reactivity
Please visit www.millipore.com/SmartFlare
RPMI-1640 Media
Microsoft Word - r7880for.doc
RPMI-1640 Media
RPMI-1640 medium was developed by Moore et al., at Roswell Park Memorial Institute, hence the acronym RPMI.
The formulation is based on the RPMI-1630 series of media utilizing
Data Sheet - T8665
3,3,5,5-Tetramethylbenzidine (TMB) Liquid Substrate System (T8665) - Datasheet
3,3,5,5-Tetramethylbenzidine (TMB) Liquid
Substrate System
peroxidase substrate
Catalog Number T8665
Storage Temperature 2–8 C
Product Description
3,3,5,5-Tetramethylbenzidine
CIC Hu-Cy3 SmartFlare™ RNA Detection Probe
Background
SPECIES LEGEND: Hu Human Ms Mouse Rt Rat Rb Rabbit WR Most Common Vertebrates ( ) Predicted Reactivity
Please visit www.millipore.com/SmartFlare
Surgical Instrument Care and Handling Instructions
World Precision Instruments
The proper care and handling of your valuable
surgical instruments will improve their
longevity and function. Choose a protocol
appropriate for your environment from these
cleaning techniques.
Care & Handling
of Surgical Instruments
061419
Tumor-Associated Antigen Panel Cell Lines for Testing of Novel Immunotherapies
–/–
Knockout
Cell Line
+/+
Parental
Cell Line
+/+
Low
+/+
High
LentivirusZFN
Tumor-Associated Antigen Panel Cell Lines
for Testing of Novel Immunotherapies
Revolutionizing Immuno-Oncology Research with Biologically-Relevant Cellular Assays
We have utilized CompoZr® zinc finger
BPLS Agar (Brilliant-green Phenol-red Lactose Sucrose, 107237 MIBIO
107237.fm
1
BPLS Agar (Brilliant-green Phenol-red Lactose Sucrose
Agar)
Selective culture medium for the isolation of Salmonella with the exception of S. typhosa and Shigella from
pathological material, faeces, urine, foodstuffs etc.
Product Information Sheet - C2538
Carboplatin (C2538) - Product Information Sheet
Carboplatin
Product Number C 2538
Store at Room Temperature
Product Description
Molecular Formula: C6H12N2O4Pt
Molecular Weight: 371.3
CAS Number: 41575-94-4
Data Sheet 1.46765
Templ_Meeting_Minutes_MilliporeSigma
1.46765.0020 Page 1 of 4
Technical Data Sheet
ReadyPlate™55 SLANETZ and BARTLEY acc. ISO 7899
Ordering number: 1.46765.0020
For the enumeration of enterococci in water and other
Water for Histology Staining - Application Note Biomedical
Application Note
Biomedical
Water for Histology Staining
Estelle Riche and Stephane Mabic
Merck, Lab Water, Saint-Quentin-en-Yvelines, France
Abstract
Water purified with a combination of reverse osmosis, electrodeionization, and ultraviolet light was used to
prepare reagents and rinsing solutions for the hematoxylin
Data Sheet
Foxx Life Sciences’ EZBio®pure Titanium
Round (TR) Media Bottles, have an enhanced the
media bottle design. To stabilize the media bottles we
lowered the bottle height, angled shoulder for smooth and
complete pouring of media, and molded finger grips to
Whatman 934-AH Glass Microfiber Filters Tech Sheet
Whatman 934-AH Glass Microfiber Filters Tech Sheet
GE Healthcare
Data file 51646 Filter papers
Whatman™ 934-AH™ Glass Microfiber filters
Whatman 934-AH Glass Microfiber filters (Fig 1) are suitable
for suspended
Data Sheet - P3360
Prostate Specific Antigen–a1-Antichymotrypsin Complex human (P3360) - Datasheet
Prostate Specific Antigen-α-1-
Antichymotrypsin Complex
Product Number P 3360
Storage Temperature –20 °C
Synonym: PSA-ACT
Data Sheet - P0060 - Lot 110M0845
PLK1, active (P0060) - Datasheet
PLK1, active, His-tagged, human
PRECISIO Kinase
recombinant, expressed in Sf9 cells
Catalog Number P0060
Lot Number 110M0845
Storage Temperature –70 °C
Synonyms: STPK13
Product Description
PLK1 is a member of the
Data Sheet - 92412
Templ_Meeting_Minutes_MilliporeSigma
Page 1 of 2
92412 Yeast Mannitol Agar without Calcium (YMA w/o Ca)
Product Information Sheet - A1075
a1075pis.doc
Amyloid β Protein Fragment 1-40
Product Number A 1075
Storage Temperature -0 °C
Product Description
Molecular Formula: C194H295N53O58S
Molecular Weight: 4,329.8 Da
CAS
RABBIT ANTI-CARBOXYPEPTIDASE H
Microsoft Word - AB5314.doc
28820 Single Oak Drive • Temecula, CA 92590
Technical Support: T: 1-800-MILLIPORE (1-800-645-5476) •
MOUSE ANTI-CYTOKERATIN 10 MONOCLONAL ANTIBODY
MOUSE ANTI-*****
28820 Single Oak Drive • Temecula, CA 92590
Technical Support: T: 1-800-MILLIPORE (1-800-645-5476) • F: 1-800-437-7502
Product Information Sheet - M3913
SigmaMarker (S8320, M3913, S8445) - Technical Bulletin
SigmaMarker
High Range, Molecular Weight 36,000−200,000 Da
Catalog Number S8320
Low Range, Molecular Weight 6,500−66,000 Da
Catalog Number M3913
Wide Range, Molecular Weight 6,500−200,000 Da
Catalog Number
Product Information Sheet - D8666
d8666bul.doc
DEOXYRIBONUCLEIC ACID (DNA) SPOOLING
EDUCATIONAL KIT
Product Number D 8666
Technical Bulletin ED-100
TECHNICAL BULLETIN
Product Description
Deoxyribonucleic acid, usually abbreviated as DNA, is
Data Sheet - S5253
Stearoyl chloride (S5253) - Datasheet
STEAROYL CHLORIDE
Product Number S 5253
The melting point of this product is approximately
22 °C. Prior to opening ampule, liquify contents
Scepter Publications (2011-present)
Scepter Publications 2013
Scepter Publications
(2011-present) Updated on May 31, 2013
Cell diameter measurements obtained with a handheld cell counter could be used
as a surrogate marker of G2/M arrest and apoptosis in
Metformin and Related Impurities Sequant®ZIC®-HILIC
HPLC Application Note: USP method - Metformin
IFU Date of Issue 2014-05 Previous Version
English
Vented Millex®-GV 0.22 µm Filter Unit
Cat. No. SLGVV255F (50/box)
● Single use only ● Sterile ● Non-pyrogenic
Intended Use
Sterilization of aqueous solutions for medical applications.
Indications for Use
Vented Millex®-GV filter units are syringe filters
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