[Skip to Content](https://www.sigmaaldrich.com#main-content)
[](https://www.sigmaaldrich.com/CZ/en)
Products
Cart0
CZEN
Products
ProductsApplicationsServicesResourcesSupport
[Login / Register](https://www.sigmaaldrich.com/oidc-sign-in)
[Order Lookup](https://www.sigmaaldrich.com/CZ/en/order-lookup)
[Quick Order](https://www.sigmaaldrich.com/CZ/en/quick-order)
Cart0
[Home](https://www.sigmaaldrich.com/CZ/en)[qPCR](https://www.sigmaaldrich.com/CZ/en/applications/genomics/qpcr)RT-qPCR Basic Troubleshooting
# RT-qPCR Basic Troubleshooting
Experiencing poor reproducibility or low signals when performing RT-qPCR? Watch our brief webinar to learn tips for troubleshooting your RT-qPCR process. Understanding the functions of RNA is crucial to gene expression, and reverse transcription is a critical step to any RNA-based workflow. This video highlights the principles of reverse transcription and quantitative PCR, and how you can incorporate this technology in to your workflow.
[Learn more](https://www.sigmaaldrich.com/CZ/en/applications/genomics/pcr) about our complete [PCR portfolio](https://www.sigmaaldrich.com/CZ/en/products/molecular-biology-and-functional-genomics/pcr-and-amplification/pcr-reagents-and-kits) and how to select the right reagents for your experiment.
__Related Articles__
- [RT-qPCR – Quantitative Reverse Transcription PCR](https://www.sigmaaldrich.com/CZ/en/technical-documents/technical-article/genomics/qpcr/rt-qpcr)
- [How qPCR Works](https://www.sigmaaldrich.com/CZ/en/technical-documents/technical-article/genomics/qpcr/how-qpcr-works)
Top
__Sign In To Continue__
To continue reading please sign in or create an account.
Sign In__Don't Have An Account?__Register
Support[Customer Support](https://www.sigmaaldrich.com/CZ/en/support/customer-support)[Contact Us](https://www.sigmaaldrich.com/CZ/en/collections/offices)[FAQ](https://maestro.my.site.com/knowledgeportal/s/)[Safety Data Sheets (SDS)](https://www.sigmaaldrich.com/CZ/en/documents-search?tab=sds)[Certificates (COA/COO)](https://www.sigmaaldrich.com/CZ/en/documents-search?tab=coa)[Quality & Regulatory](https://www.sigmaaldrich.com/CZ/en/life-science/quality-and-regulatory-management)[Calculators & Apps](https://www.sigmaaldrich.com/CZ/en/support/calculators-and-apps)[Webinars](https://www.sigmaaldrich.com/CZ/en/collections/webinars)
Orders[Order Lookup](https://www.sigmaaldrich.com/CZ/en/order-lookup)[Quick Order](https://www.sigmaaldrich.com/CZ/en/quick-order)[Custom Products](https://www.sigmaaldrich.com/CZ/en/services/custom-products)[eCommerce Solutions](https://www.sigmaaldrich.com/CZ/en/life-science/ecommerce/ecommerce-solutions)
Company[About Us](https://www.sigmaaldrich.com/CZ/en/life-science/about-us)[Responsibility](https://www.sigmaaldrich.com/CZ/en/life-science/ssbi)[Events](https://www.sigmaaldrich.com/CZ/en/collections/events)[Press Releases](https://www.sigmaaldrich.com/CZ/en/collections/press)[Programs](https://www.sigmaaldrich.com/CZ/en/life-science/partnership-programs)[Careers](https://careers.merckgroup.com/global/en)[Offices](https://www.sigmaaldrich.com/CZ/en/collections/offices)
Social Media
[](https://www.linkedin.com/company/merck-life-science)
[](https://x.com/Merck_lifesci)
[](https://www.facebook.com/Merck.lifescience)
[](https://www.instagram.com/mercklifescience/)
[](https://www.youtube.com/@MerckLifeScience/featured)
Merck
__Research. Development. Production.__
We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.
[](https://www.sigmaaldrich.com/CZ/en)
[Sigma-Aldrich® Solutions](https://www.sigmaaldrich.com/CZ/en/life-science/sigma-aldrich)[BioReliance® Solutions](https://www.sigmaaldrich.com/CZ/en/life-science/bioreliance)[Millipore® Solutions](https://www.sigmaaldrich.com/CZ/en/life-science/millipore)[SAFC® Solutions](https://www.sigmaaldrich.com/CZ/en/life-science/safc)[Milli-Q® Solutions](https://www.sigmaaldrich.com/CZ/en/life-science/milliq)[Supelco® Solutions](https://www.sigmaaldrich.com/CZ/en/life-science/supelco)
© 2026 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved, including Text and Data Mining for AI training and similar technologies.
Reproduction of any materials from the site is strictly forbidden without permission.
[Site Use Terms](https://www.sigmaaldrich.com/CZ/en/life-science/legal/site-use-terms)|[Privacy Policy](https://www.sigmaaldrich.com/CZ/en/life-science/legal/privacy-statement)|[General Terms and Conditions of Sale](https://www.sigmaaldrich.com/CZ/en/life-science/legal/terms-and-conditions)|[Copyright Consent](https://www.sigmaaldrich.com/CZ/en/life-science/legal/copyright-consent) |[Site Map](https://www.sigmaaldrich.com/CZ/en/site-map)
An unknown error has occured.