[Skip to Content](https://www.sigmaaldrich.com#main-content) [](https://www.sigmaaldrich.com/CZ/en) Products Cart0 CZEN Products ProductsApplicationsServicesResourcesSupport [Login / Register](https://www.sigmaaldrich.com/oidc-sign-in) [Order Lookup](https://www.sigmaaldrich.com/CZ/en/order-lookup) [Quick Order](https://www.sigmaaldrich.com/CZ/en/quick-order) Cart0 [Home](https://www.sigmaaldrich.com/CZ/en)[qPCR](https://www.sigmaaldrich.com/CZ/en/applications/genomics/qpcr)Probe-based qPCR # Probe-based qPCR Probe-based qPCR functions by recognition of a specific sequence on the desired PCR product. Unlike SYBR® Green qPCR methods, that use a intercalating dye to bind all double-stranded DNA, probe-based qPCR uses fluorescent-labeled target-specific probes. This technique yields increased specificity and sensitivity since only specific DNA molecules will be labeled. Other fluorescent dyes can also be combined with probe-based qPCR to label, identify, amplify, and quantify various sequences. Our probe-based qPCR reagents are provided as ReadyMixes with hot-start activity to promote higher specificity and sensitivity. Our LuminoCt® products are prepared optimized for common qPCR instruments and our KiCqStart® reagents are designed for a range of qPCR instruments. Fast/Conventional qPCR: - [KiCqStart®](https://www.sigmaaldrich.com#1) – Formats for all major qPCR instruments - [LuminoCt®](https://www.sigmaaldrich.com#2) – Compatible with most cyclers Conventional qPCR - [JumpStart](https://www.sigmaaldrich.com#3) ## Fast/Conventional qPCR ### [](https://www.sigmaaldrich.com)KiCqStart® ReadyMix™ – Instrument Optimized KiCqStart® Probe PCR features a stringent hot-start mechanism for greater specificity. The mastermix contains all required basic components for qPCR; simply add primers, probe, and water to finish preparing your assay. KiCqStart® is compatible with various fluorescent probes including TaqMan® probes, Molecular Beacons, LightCycle® Probes, and Scorpion® Probes. Select the KiCqStart® product below that is optimized for your instrument!  Sorry, an unexpected error has occurred Response not successful: Received status code 500 ## [](https://www.sigmaaldrich.com)LuminoCt® ReadyMixes – Highly Optimized LuminoCt® performs fast qPCR in as little as 25 minutes without sacrificing accuracy, precision, or sensitivity. It is produced as a highly optimized ReadyMix developed with JumpStart Taq antibody for rapid activation and unparalleled sensitivity. The ReadyMix virtually eliminates the need to optimize assay conditions and is compatible with commonly available qPCR instruments. Often users are able to simply purchase the kit, build their assay, and get right to work.  Sorry, an unexpected error has occurred Response not successful: Received status code 500 ## Conventional qPCR ### [](https://www.sigmaaldrich.com)JumpStart Taq ReadyMix for Conventional qPCR Our JumpStart ReadyMixes are ideal for high throughput; they contain all necessary components for qPCR, simply combine it with fluorescent detection, primers, and template. Unlike other pre-incorporated products, JumpStart Taq ReadyMix allows for maximum flexibility in choosing your own detection method since none is incorporated.  Sorry, an unexpected error has occurred Response not successful: Received status code 500 __Related Articles__ - [PCR/qPCR/dPCR Assay Design](https://www.sigmaaldrich.com/CZ/en/technical-documents/protocol/genomics/pcr/pcr-qpcr-dpcr-assay-design) - [Mgat4 May Play a Role in Increased Sialylation by Overexpressing Functional MGAT1 in Mgat1-Disrupted Chinese Hamster Ovary (CHO) Cells](https://www.sigmaaldrich.com/CZ/en/technical-documents/technical-article/genomics/cloning-and-expression/mgat1-disrupted-chinese-hamster-ovary-cells) - [Complete Solutions for PCR Assay Development](https://www.sigmaaldrich.com/CZ/en/technical-documents/technical-article/genomics/dna-and-rna-purification/pcr-assay-development) - [Water for Next-Generation Sequencing](https://www.sigmaaldrich.com/CZ/en/technical-documents/technical-article/genomics/next-gen-sequencing/water-for-next-generation-sequencing) - [PCR Assay Optimization and Validation](https://www.sigmaaldrich.com/CZ/en/technical-documents/technical-article/genomics/pcr/assay-optimization-and-validation) - [DNA Oligonucleotide Synthesis](https://www.sigmaaldrich.com/CZ/en/technical-documents/technical-article/genomics/pcr/dna-oligonucleotide-synthesis) - [Locked Nucleic Acid & Minor Groove Binder / Eclipse Dark Quencher](https://www.sigmaaldrich.com/CZ/en/technical-documents/technical-article/genomics/pcr/locked-nucleic-acids-faq) - [PCR Technologies Guide](https://www.sigmaaldrich.com/CZ/en/technical-documents/technical-article/genomics/pcr/pcr-technologies-protocols-introduction) - [View More](https://www.sigmaaldrich.com/CZ/en/search/facet-search?focus=sitecontent&term=facet-search) Top __Sign In To Continue__ To continue reading please sign in or create an account. 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