[Skip to Content](https://www.sigmaaldrich.com#main-content) [](https://www.sigmaaldrich.com/US/en) Products Cart0 USEN Products ProductsApplicationsServicesResourcesSupport [Login / Register](https://www.sigmaaldrich.com/oidc-sign-in) [Order Lookup](https://www.sigmaaldrich.com/US/en/order-lookup) [Quick Order](https://www.sigmaaldrich.com/US/en/quick-order) Cart0 [Home](https://www.sigmaaldrich.com/US/en)[Small Molecule HPLC](https://www.sigmaaldrich.com/US/en/applications/analytical-chemistry/liquid-chromatography/small-molecule-hplc)Supel™ Carbon LC U/HPLC Columns # Supel™ Carbon LC U/HPLC Columns Porous graphitic carbon (PGC) particles, designed by a unique and patented synthetic process, constitute the packing of Supel™ Carbon LC U/HPLC columns. The key advantages of using PGC columns over silica particle-packed columns include: - __Elevated Temperature Stability:__ Columns can be readily operated at temperatures up to 250 °C, thereby allowing faster and more efficient separations. - __pH Stability:__ Compatible with mobile phases in the pH range of 1 – 14 at any temperature without causing a decline in the column lifespan. - __Unique Retention Mechanism:__ The Polar Retention Effect on Graphite (PREG) mechanism allows for the retention of polar or charged compounds without hydrophilic interaction liquid chromatography (HILIC) conditions. The mechanism also allows the resolution of geometric isomers. - __Compatibility with Any Solvent:__ Any polar or non-polar solvent can be used for the resolution of an analyte of interest. - __Pressure Stability up to 700 bar.__ ### Supel™ Carbon LC Specifications Particle PlatformPorous Graphitic Carbon (PGC) Particle Size2.7 µM Pore Size200 Å Surface Area155 m2/g pH Range1-14 Maximum Temperature250 °C The Supel™ Carbon LC columns are available in internal diameters of 2.1, 3.0, and 4.6 mm with guard cartridges and holders available for each inner diameter.  __Figure 1.__ Scanning electron microscope (SEM) image of the Supel™ Carbon LC particles. Notice the narrow particle size distribution The patented synthetic process used for the synthesis of Supel™ Carbon LC columns generates a narrow particle size distribution (PSD). The resulting narrow PSD leads to a higher efficiency due to the reduction in brand broadening caused by eddy diffusion effects. One application demonstrating the Supel™ Carbon LC’s excellent resolving power is the separation of Vitamins D2 and D3 metabolites. These metabolites are polar and structurally similar, making their chromatographic separation difficult on the conventional silica based reversed-phase columns. The Supel™ Carbon LC column resolves the metabolites with high efficiency. ## Resolution of Vitamin D2 and D3 Metabolites on Supel™ Carbon LC Column  __Figure 2.__Chromatographic separation of Vitamin D2 and D3 metabolites on Supel™ Carbon LC. Conditions: Column: Supel™ Carbon LC, 10 cm x 2.1 mm I.D., 2.7 µM; Mobile Phase: \[A] 2-Propanol; \[B] Tetrahydrofuran; Gradient: 0% B to 70% B in 15 min; hold at 70% B for 5 min; Flow Rate: 0.3 mL/min; Column Temp.: 25 °C; Detector: UV, 275 nm; Injection: 2.0 µL; Sample: Vitamin D2 and D3 metabolites mix, varied concentration, ethanol | | | | |-------|---------------------------------------|----------------------| | Peaks | Compound | Retention Time (min) | | 1 | 3-epi-25-hydroxyvitamin D3 (50 µg/mL) | 8.294 | | 2 | 25-hydroxyvitamin D3 (25 µg/mL) | 9.125 | | 3 | 3-epi-25-hydroxyvitamin D2 (50 µg/mL) | 11.126 | | 4 | 25-hydroxyvitamin D2 (100 µg/mL) | 12.052 | Table 1.Peak Identities of Vitamin D2 and D3 Metabolites on Supel™ Carbon LC Column. Supel™ Carbon LC columns are compatible with all HPLC, UHPLC, UPLC, and LC-MS instruments.   ## Materials Sorry, an unexpected error has occurred Response not successful: Received status code 500 ## Related Documents [White Paper: Retention Behaviour of N-Acetyl Hexosamine Isomers On A Supel™ Carbon LC Column](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/378/543/retention-behaviour-n-acetyl-hexosamine-supel-carbon-lc-wp13272en-ms.pdf) [Flyer: Download Carbon Column](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/320/767/carbon-column-fl7038en-ms.pdf) [Application Note: Determination of Glyphosate, Glufosinate, and AMPA in Green Tea by LC-MS/MS with QuEChERS Cleanup](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/663/670/determination-glyphosate-glufosinate-ampa-green-tea-an14162en-ms.pdf) [Application Note: LC-MS/MS Analysis of 20 Underivatized Amino Acids on Supel™ Carbon LC Column](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/832/649/underivatized-amino-acids-an6469en-ms.pdf) [Application Note: UHPLC-MS/MS Analysis of Polar Pesticides on the Supel™ Carbon LC Column](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/305/089/supel-carbon-polar-pesticides-an6679en-ms.pdf) [Application Note: UHPLC Analysis of Vitamin D2 & D3 Metabolites and Epimers on Supel™ Carbon LC Column](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/455/783/uhplc-analysis-vitamin-d-an6468en-ms.pdf) [Application Note: UHPLC-MS Analysis of Released N-Glycans from Human IgG on a Supel™ Carbon LC Column](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/409/895/supel-carbon-lc-n-glycan-human-igg-an11781en-ms.pdf) __Related Articles__ - [HPLC Separation: Ginsenosides from American Ginseng](https://www.sigmaaldrich.com/US/en/technical-documents/protocol/analytical-chemistry/small-molecule-hplc/separation-ginsenosides) - [Determination of Formaldehyde and Acetaldehyde in Air Using DNPH Cartridges and Automated On-Line Desorption Followed by HPLC](https://www.sigmaaldrich.com/US/en/technical-documents/protocol/environmental-testing-and-industrial-hygiene/air-testing/aldehydes-air-dnph) - [Cannabinoid CRMs: Testing Accuracy & Traceability](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/analytical-chemistry/calibration-qualification-and-validation/cannabinoid-certified-reference-materials-improved-testing-accuracy-traceability) - [Solution with 17 Amino Acids as TraceCERT® Certified Reference Material](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/analytical-chemistry/calibration-qualification-and-validation/certified-amino-acid-mix-solution) - [Extractables Studies of Single-Use Equipment Immediate Identification and Quantification of Unknown Extractables by GC/MS with a Certified Reference Material Mix for Extractables and Leachables](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/analytical-chemistry/calibration-qualification-and-validation/extractables-studies-of-single-use-equipment) - [Isotopically Labeled Steroid Standards](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/analytical-chemistry/calibration-qualification-and-validation/labeled-steroids) - [Reference Standards for Analyzing Polyphenol Catechins](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/analytical-chemistry/calibration-qualification-and-validation/reference-standards) - [A review of extractables and leachables](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/analytical-chemistry/calibration-qualification-and-validation/review-of-extractables-and-leachables) - [View More](https://www.sigmaaldrich.com/US/en/search/facet-search?focus=sitecontent&term=facet-search) Top __Sign In To Continue__ To continue reading please sign in or create an account. 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