60142

Kaliumchlorid -Lösung

BioUltra, for molecular biology, ~1 M in H₂O

• Synonym(e): KCl, potassium chloride
• Lineare Formel: KCl
• CAS-Nummer: 7447-40-7
• Molekulargewicht: 74.55
• Beilstein: 1711999
• MDL-Nummer: MFCD00011360
• UNSPSC-Code: 12352302
• PubChem Substanz-ID: 57651726
• NACRES: NA.26

Eigenschaften

• Qualität: for molecular biology
• Produktlinie: BioUltra
• Konzentration: ~1 M in H₂O
• Verunreinigungen: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected; ≤0.001% total nitrogen (N)
• pH-Wert: 5.0-8.0 (25 °C, 1 M in H₂O)
• Dichte: 1.04 g/mL at 20 °C
• Anionenspuren: sulfate (SO₄^2-): ≤10 mg/kg
• Kationenspuren: Al: ≤1 mg/kg; Ba: ≤1 mg/kg; Bi: ≤1 mg/kg; Ca: ≤5 mg/kg; Cd: ≤1 mg/kg; Co: ≤1 mg/kg; Cr: ≤1 mg/kg; Cu: ≤1 mg/kg; Fe: ≤1 mg/kg; Li: ≤1 mg/kg; Mg: ≤1 mg/kg; Mn: ≤1 mg/kg; Mo: ≤1 mg/kg; Na: ≤20 mg/kg; Ni: ≤1 mg/kg; Pb: ≤1 mg/kg; Sr: ≤1 mg/kg; Zn: ≤1 mg/kg
• λ: 1 M in H₂O
• UV-Absorption: λ: 260 nm A_max: 0.02; λ: 280 nm A_max: 0.01
• SMILES String: [Cl-].[K+]
• InChI: 1S/ClH.K/h1H;/q;+1/p-1
• InChIKey: WCUXLLCKKVVCTQ-UHFFFAOYSA-M

Beschreibung

Allgemeine Beschreibung

Potassium is an intracellular cation with just 2% of total body K⁺ inhabiting the extracellular fluid. The distribution of K⁺ between the intracellular and extracellular space is analyzed by the action of several transport pathways like Na⁺-K⁺-ATPase, the Na⁺-K⁺-2Cl-cotransporter NKCC1, the four KCCs, and a plethora of K⁺ channels. Also, skeletal muscle comprises almost 75% of total body potassium and elicits considerable influence on extracellular K⁺.[1] Potassium chloride is consumed orally to prevent or correct potassium depletion caused by diuretic use or other conditions. It is used as a food supplement and salt substitute.[2]

Anwendung

Potassium chloride solution has been used as a component of:

• multiple annealing and looping based amplification cycles (MALBAC) lysis buffer to digest proteins for single-cell multiomics sequencing[3]
• single-cell tagged reverse transcription (STRT) buffer, and STRT-RT buffer for single-cell RNA-sequencing[4]
• phosphate buffer saline (PBS) to wash transfected cells[5]

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves