Heat inactivation is required based on the specific application or protocol. It is typically necessary for hybridoma cultures or immunological studies that require the inactivation of complement proteins. However, heat inactivation is not required for all applications.
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F2442
Fetales Kälberserum
USA origin, sterile-filtered, suitable for cell culture, suitable for hybridoma
Synonym(e):
FBS, FCS, Seren, Serum
zur Ansicht der Organisations- und Vertragspreise.
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Über diesen Artikel
Biological source:
bovine fetus
Origin:
USA origin
Sterility:
sterile-filtered
Shipped in:
dry ice
Preise und Verfügbarkeit sind derzeit nicht verfügbar.
Technischer Dienst
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Unterstützung erhaltenbiological source
bovine fetus
Quality Segment
sterility
sterile-filtered
product line
FBS Classic
composition
Bovine IgG, ≤1 mg/mL , Hemoglobin, ≤20 mg/dL
origin
USA origin
technique(s)
cell culture | hybridoma: suitable, cell culture | mammalian: suitable
impurities
≤10 EU/mL endotoxin
shipped in
dry ice
storage temp.
−20°C
General description
Fetales Kälberserum (FBS) ist eine komplexe Mischung aus Biomolekülen mit geringer und hoher Molekülmasse, die optimale wachstumsfördernde und wachstumsunterdrückende Funktionen aufweist. FBS enthält Moleküle wie Wachstumsfaktoren, Proteine, Vitamine, Spurenelemente, Hormone usw. Diese Moleküle sind wichtig für das Wachstum und die Erhaltung von Zellen. Es ist eine häufig verwendete Ergänzung für Zellkulturmedien. FBS wird aus Kälberblut gewonnen. Es ist frei von Fibrin und Gerinnungsfaktoren. Die Hitzeinaktivierung von FBS blockiert das Komplementsystem. FBS aus geronnenem Blut ist die am häufigsten verwendete undefinierte Ergänzung in eukaryotischen Zellkulturen, insbesondere von Säugetieren.[1] Es wird in der Regel mit den klassischen Medien verwendet, die in den 1950ern und 1960ern entwickelt wurden. Dazu zählen viele gängige Medien wie Minimal Essential Medium (MEM),[2] Dulbecco′s Modified Eagle‘s Medien (DME, DMEM), Iscove′s Modified Eagle‘s Medium (IMDM),[3] M199[4] und Roswell Park Memorial Institute Medium, RPMI-1640.[2]
Application
Biochem/physiol Actions
FBS bleibt eine beliebte Medienergänzung, da es ein breites Spektrum von Funktionen in Zellkulturen bereitstellt. FBS liefert Nähstoffe, Wachstums- und Bindungsfaktoren und schützt die Zellen durch Mechanismen, die in serumfreien Medien(SFM)-Systemen schwer zu reproduzieren sind, vor oxidativen Schäden und Apoptose.
Analysis Note
- Auf Bakterien, Viren und Mykoplasmen getestet
- Unter aseptischen Bedingungen dreifach durch eine 0,1-μm-Membran filtriert.
- Endotoxin- und Hämoglobin-getestet
Other Notes
Auf das Vorhandensein von Bakterien, Viren, Mykoplasma, Endotoxine und auf den Hämoglobingehalt geprüft. Unter aseptischen Bedingungen dreifach durch eine 0,1-μm-Membran filtriert.
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Dieser Artikel | |||
|---|---|---|---|
| biological source bovine fetus | biological source bovine fetus | biological source bovine fetus | biological source bovine fetus |
| sterility sterile-filtered | sterility - | sterility sterile-filtered | sterility γ-irradiated, sterile-filtered |
| origin USA origin | origin USA origin | origin USA origin | origin non-USA origin |
| technique(s) cell culture | hybridoma: suitable, cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | hybridoma: suitable, cell culture | insect: suitable, cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
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Lagerklasse
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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is heat inactivation necessary?
1 Antwort-
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How to heat inactivate this serum Fetal Bovine Serum USA origin,(Cat Log - F2442)
1 Antwort-
Heat inactivation occurs when serum is heated to 56°C for a period of 30 minutes.
Here's a protocol for heat inactivation:
1. Allow serum containers to come to room temperature. Swirl bottles of serum immediately before adding to the water bath.
2. A water-filled control bottles (T1) should be placed in a 56 °C water bath. Place the containers in the water bath up to the serum line. Do not completely submerge the containers. When the temperature of T1 reaches 56 °C, start the timer set for 30 minutes. Do not allow temperatures to exceed 56 °C. Higher temperatures will denature the proteins, gel the serum or alter the performance of the serum.
3. Gently swirl the bottles every 5 to 10 minutes while in the water bath and check the temperature in the control bottle. It is not uncommon for plastic containers to bow slightly after heat treatment. This is not harmful to the serum.
4. At the end of 30 minutes remove the serum bottles from the water bath. Aliquot the serum into smaller volumes and freeze. Sigma-Aldrich does not recommend repeated thaw-freeze cycles.Hilfreich?
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is this Heat Inactivate FBS?
1 Antwort-
This product has not been heat inactivated.
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can F2442 FBS be used for culturing peritoneal macrophages? what is the suitable one?
1 Antwort-
This product is tested for suitability in mammalian cell culture. While it is not specifically tested on macrophages, it is subject to stringent specifications, including low endotoxin, and should perform well. Please see the link below to review a sample Certificate of Analysis:
https://www.sigmaaldrich.com/coa/SIGMA/F2442/23A317Hilfreich?
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Can this f2442 fbs be used for culture expansion of mesenchymal stem cells? Please confirm Thanks
1 Antwort-
Fetal Bovine Serum (Cat. no.: F2442) is suitable for use in mammalian cell culture. However, it is not tested specifically in mesenchymal stem cell culture. There is some evidence in the literature that this product would be suitable. See the link below for a relevant reference:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4545441/Hilfreich?
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What is the difference between Fetal Bovine Serum F2442 and 12103C?
1 Antwort-
Product No. 12103C meets most of the specifications of F2442. Both products are from the same raw material suppliers and undergo the same stringent testing prior to release. However, the F2442 has been use-tested in hybridoma culture, whereas 12103C has not.
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Does Sigma-Aldrich offer fetal bovine serum (FBS) that is antibiotic-free?
1 Antwort-
FBS can be tested for the presence of antibiotics. Products F2442, F0926, and F6178, Fetal Bovine Serum are routinely tested for Tetracycline (by far the most commonly used antibiotic in animals) and the results are reported on the lot specific Certificate of Analysis.Per the USDA, Antibiotics may be given to prevent or treat disease in cattle. A "withdrawal" period is required from the time antibiotics are administered until it is legal to slaughter the animal. This is so residues can exit the animal's system. FSIS randomly samples cattle at slaughter and tests for residues. Data from this Monitoring Plan have shown a very low percentage of residue violations.The USDA does random sampling of all cattle submitted for slaughter in USDA inspected facilities for antibiotics. The mandatory withdrawal period is 24 hours. Based on withdrawal periods required by the USDA, animal testing at slaughter and finished product testing, our serum is antibiotic free.
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Are all Fetal Bovine Serum (FBS) products heat inactivated?
1 Antwort-
Not all Fetal Bovine Serum products are heat inactivated. Product numbers F4135 and 12306C are heat-inactivated FBS.
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How is Fetal Bovine Serum, Product F2442, cell culture tested?
1 Antwort-
Biological performance is assessed using Sigma cell culture medium supplemented with the serum and tested at a final concentration of 10%. Seeding efficiency, doubling time, and final cell density are measured on appropriate cell lines. Growth studies are carried through two subculture generations and cell growth is plotted as a logarithmic function of time in culture. During the testing period, cultures are examined microscopically for atypical morphology and evidence of cytotoxicity. Hybridoma -tested serum has been shown to support mouse myeloma cells and hybridoma cells in culture.
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What is the Department of Transportation shipping information for this product?
1 Antwort-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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