To reconstitute dissolve G6378, Glucose-6-phosphate Dehydrogenase, in water and a dilute buffer. Following reconstitution, it should be refrigerated at 4°C, with stock solutions remaining stable for up to 6 months at this temperature.
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Wichtige Dokumente
G6378
Glucose-6-phosphat-Dehydrogenase aus Backhefe (S. cerevisiae)
Type XV, lyophilized powder, 200-400 units/mg protein (modified Warburg-Christian)
Synonym(e):
G-6-P-DH, Zwischenferment
Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
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100 UNITS
64,10 €
250 UNITS
131,00 €
500 UNITS
240,00 €
1000 UNITS
477,00 €
2000 UNITS
823,00 €
5000 UNITS
1.670,00 €
Alle Fotos(5)
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Ansicht ändern
100 UNITS
64,10 €
250 UNITS
131,00 €
500 UNITS
240,00 €
1000 UNITS
477,00 €
2000 UNITS
823,00 €
5000 UNITS
1.670,00 €
About This Item
Empfohlene Produkte
Typ
Type XV
Qualitätsniveau
Form
lyophilized powder
Spezifische Aktivität
200-400 units/mg protein (modified Warburg-Christian)
Mol-Gew.
128 kDa
Aufgereinigt durch
crystallization
Anwendung(en)
diagnostic assay manufacturing
Versandbedingung
dry ice
Lagertemp.
−20°C
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Allgemeine Beschreibung
Anwendung
Glucose-6-Phosphat-Dehydrogenase wird verwendet, um die Aktivität von Ketose-Reduktase in sich entwickelndem Mais-Endosperm zu testen.[3]
Biochem./physiol. Wirkung
Glucose-6-Phosphat-Dehydrogenase katalysiert die Umwandlung von Glucose-6-Phosphat in 6-Phosphogluconolaceton und damit den ersten Schritt des Pentose-Phosphat-Wirkungsweges<<<17>>>.
Glucose-6-Phosphat-Dehydrogenase katalysiert die Umwandlung von Glucose-6-Phosphat in 6-Phosphogluconolaceton und damit den ersten Schritt des Pentose-Phosphat-Wirkungsweges.
Einheitendefinition
Eine Einheit oxidiert in Gegenwart von NADP bei pH-Wert 7,4 und 25 °C pro Minute 1,0 μmol D-Glucose-6-Phosphat zu 6-Phospho-D-Gluconat.
Physikalische Form
Lyophilisiert und im Wesentlichen sulfatfrei; enthält ca. 20 % Natriumcitrat bezogen auf das Trockengewicht
Angaben zur Herstellung
Hergestellt aus G7877
Signalwort
Danger
H-Sätze
P-Sätze
Gefahreneinstufungen
Resp. Sens. 1
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Persönliche Schutzausrüstung
Eyeshields, Gloves, type N95 (US)
Hier finden Sie alle aktuellen Versionen:
Analysenzertifikate (COA)
Lot/Batch Number
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Yong Yang et al.
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Mycobacteria spontaneously form surface-associated multicellular communities, called biofilms, which display resistance to a wide range of exogenous stresses. A causal relationship between biofilm formation and emergence of stress resistance is not known. Here, we report that activation of a nitrogen
K E Reilly et al.
Biochemical and biophysical research communications, 216(3), 993-998 (1995-11-22)
A commercial preparation of glucose-6-phosphate dehydrogenase (G6PD) purified from Saccharomyces cerevisiae was subjected to PAGE analysis under both nondenaturing and denaturing conditions. The enzyme, identified by both activity staining and anti-yeast G6PD antibody immunoblotting, was shown to contain carbohydrate using
D C Doehlert
Plant physiology, 84(3), 830-834 (1987-07-01)
Ketose reductase (NAD-dependent polyol dehydrogenase EC 1.1.1.14) activity, which catalyzes the NADH-dependent reduction of fructose to sorbitol (d-glucitol), was detected in developing maize (Zea mays L.) endosperm, purified 104-fold from this tissue, and partially characterized. Product analysis by high performance
P Andrews
The Biochemical journal, 96(3), 595-606 (1965-09-01)
1. Correlation between elution volume, V(e), and molecular weight was investigated for gel filtration of proteins of molecular weights ranging from 3500 (glucagon) to 820000 (alpha-crystallin) on Sephadex G-200 columns at pH7.5. 2. Allowing for uncertainties in the molecular weights
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A combination of biological control and host plant resistance would be desirable for optimally controlling the greenhouse whitefly, Trialeurodes vaporariorum in tomato crops. Whitefly settlement preference, oviposition, and survivorship were evaluated on ABL 10-4 and 'Moneymaker', two nearly-isogenic tomato lines
Protokolle
To measure glucose-6-phosphate dehydrogenase activity, beta-nicotinamide adenine dinucleotide phosphate is used in a spectrophotometric rate determination assay at 340 nm.
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What are the recommended reconstitution and storage procedures for item G6378, Glucose-6-phosphate Dehydrogenase from baker's yeast (S. cerevisiae)?
1 Antwort-
Hilfreich?
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What is the activity and protein content of the lot number of Product G6378, Glucose-6-phosphate Dehydrogenase, I have?
1 Antwort-
The protein content and the specific activity of the enzyme can be found on the lot-specific certificate of analysis.
Hilfreich?
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What does Type XV mean in the description of Product G6378, Glucose-6-phosphate Dehydrogenase?
1 Antwort-
The type designation Type XV is a Sigma in-house designation and does not represent any kind of biochemical significance. It simply refers to the order of addition of the product to the catalog (i.e., first there was a type I, then a type II, etc.).
Hilfreich?
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What are the Km values for Product G6378, Glucose-6-phosphate Dehydrogenase?
1 Antwort-
The Km values for glucose 6-phosphate and NADP are 2.0 x 10-5 and 2.0 x 10-6 , respectively in Tris buffer, pH 8.0 containing 10 mm magnesium chloride at 38°C.
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What is the Department of Transportation shipping information for this product?
1 Antwort-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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