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GHS132

Sigma-Aldrich

Hämatoxylin-Lösung, Gill 1

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1 L
95,30 €

95,30 €

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1 L
95,30 €

About This Item

MDL-Nummer:
MFCD00078111
UNSPSC-Code:
41116124
PubChem Substanz-ID:
24895389
NACRES:
NA.47

95,30 €

Versandbereit am17. April 2025Details

Bulk-Bestellung anfordern

Form

solution

Haltbarkeit

Expiry date on the label.

IVD

for in vitro diagnostic use

Konzentration

2 g/L

Methode(n)

microbe id | staining: suitable

pH-Wert

2.5-3.5

Anwendung(en)

hematology
histology

Lagertemp.

room temp

SMILES String

Oc1cc2C[C@@]3(O)COc4c(O)c(O)ccc4[C@H]3c2cc1O

InChI

1S/C16H14O6/c17-10-2-1-8-13-9-4-12(19)11(18)3-7(9)5-16(13,21)6-22-15(8)14(10)20/h1-4,13,17-21H,5-6H2/t13-,16+/m0/s1

InChIKey

WZUVPPKBWHMQCE-XJKSGUPXSA-N

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Verwandte Kategorien

Anwendung

Die Formulierung nach Gill I wird zur progressiven Färbung in der Zytologie verwendet. Zur Verwendung bei Hämatoxylin-Eosin-Färbeverfahren.

Sonstige Hinweise

2 g/l zertifiziertes Hämatoxylin

Piktogramme

Health hazardExclamation mark
GHS08,GHS07

Signalwort

Warning

H-Sätze

H302,H373

Gefahreneinstufungen

Acute Tox. 4 Oral - STOT RE 2 Oral

Zielorgane

Kidney

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number
0000358242
SLCQ2986
0000358242
SLCL2352
SLCH6216

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Die Dokumentenbibliothek aufrufen

Tiziana Annese et al.
Histochemistry and cell biology, 153(3), 185-192 (2019-12-18)
Diffuse large B-cell lymphomas (DLBCLs) are the most common types of Non-Hodgkin's lymphomas and are highly heterogeneous in terms of phenotype and treatment response. The natural course of DLBCLs tumor progression is featured by a flow of events leading to
P J De Andrés et al.
Reproduction in domestic animals = Zuchthygiene, 51(2), 269-275 (2016-02-24)
Canine inflammatory mammary cancer (IMC) has been proposed as a model for the study of human inflammatory breast cancer (IBC). The aims of this study were to compare the immunohistochemical expression of aromatase (Arom) and several hormone receptors [estrogen receptor
Arinjay Banerjee et al.
Scientific reports, 10(1), 7257-7257 (2020-05-01)
Coronaviruses that cause severe acute respiratory syndrome (SARS) and Middle East respiratory syndrome (MERS) are speculated to have originated in bats. The mechanisms by which these viruses are maintained in individuals or populations of reservoir bats remain an enigma. Mathematical
Patrick P van Vliet et al.
Developmental biology, 429(1), 249-259 (2017-07-04)
Correct cardiac development is essential for fetal and adult life. Disruptions in a variety of signaling pathways result in congenital heart defects, including outflow and inflow tract defects. We previously found that WNT11 regulates outflow tract development. However, tissue specific
Nathan Ungerleider et al.
PLoS pathogens, 14(8), e1007206-e1007206 (2018-08-07)
Our appreciation for the extent of Epstein Barr virus (EBV) transcriptome complexity continues to grow through findings of EBV encoded microRNAs, new long non-coding RNAs as well as the more recent discovery of over a hundred new polyadenylated lytic transcripts.
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Verwandter Inhalt

Zytologie & Zytodiagnostik

Kurzinformation zum Gebiet der Zytologie, ihrer Rolle bei der Zytodiagnostik und zum Einsatz von Standard- und Spezialfärbungen für die mikroskopische Analyse zum Nachweis von Zellanomalien.

Cytology & Cytodiagnosis

Brief information on the field of cytology, its role in cytodiagnosis and the use of standard and special stains for microscopic analysis to detect cellular abnormalities.

Fragen

1-2 von 2 Fragen  

  1. When using SLCH6216, there is light purple/violet staining of nuclei with a gray background in the cytoplasm. Increasing the staining time up to 15 minutes does not result in increased stain intensity. How can this issue be addressed?

    1 Antwort

    1. When the Gill No 1 hematoxylin is first poured from the bottle, the solution appears as a dark reddish-purple color. If the nuclei appear purple instead of blue after staining, it suggests that the slides were not adequately blued. The Instructions for Use advise using Scott's Tap Water Substitute (catalog number S5134). If warm running tap water was used, it's likely that the slides were not washed for a sufficient duration to convert the purple color to blue. If Scott's Tap Water Substitute was used, it's possible that the old solution needs to be replaced with a freshly diluted 1X solution from the 10X concentrate. Although the official recommendation is for Scott's Tap Water Solution, other blueing solutions are commonly used with different hematoxylin solutions. For instance, 15 grams of sodium bicarbonate per liter of tap or deionized water can be used, or a saturated solution of lithium carbonate. Some have also used a few drops of concentrated ammonium hydroxide in 500 ml of tap or deionized water in the past, but it's important to avoid using too much as it can strip the tissue sections from the glass slides. Typically, the slides are left in the blueing solution until the tissue sections appear blue and not purple. Once the tissue has turned blue, it should be thoroughly washed in running tap water to remove any traces of the blueing solution. Proper removal of the blueing solution is crucial, as leaving traces on the slides will lead to poor staining with Eosin Y solution.

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  2. 혹시 해당 제품의 대체품을 추천해 주실 수 있을까요?

    1 Antwort

    1. The direct replacements for GHS132(1L) would be GHS316 (500mL) and GHS1128(4L). There are other similar formulations that may be of interest. See the list below. Each option includes a link to the product datasheet and the product list for that formulation, respectively.
      1. Gill No. 2
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/620/308/procedure-ghs-hematoxylin-stains-ms.pdf
      https://www.sigmaaldrich.com/US/en/substance/hematoxylinsolutiongillno21234598765
      2. Gill No. 3
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/620/308/procedure-ghs-hematoxylin-stains-ms.pdf
      https://www.sigmaaldrich.com/US/en/substance/hematoxylinsolutiongillno31234598765
      3. Mayer's Hematoxylin
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/317/066/procedure-mhs-mayers-hematoxylin-solution-ms.pdf
      https://www.sigmaaldrich.com/US/en/substance/mayershematoxylinsolution1234598765
      4. Harris Hematoxylin
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/239/767/procedure-hhs-harrishematoxylinsolution-ms.pdf
      https://www.sigmaaldrich.com/US/en/substance/harrishematoxylinsolutionmodified1234598765

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