Based on previous results, a 20% difference in methylation is typically observable. It is recommended to conduct replicate experiments, assess the margin of error (technical and/or biological), and utilize a p-test or other statistical approach to evaluate its applicability in your specific case.
MDQ1
Imprint® methyliertes DNA-Quantifizierungskit
To measure global DNA methylation shifts from as low as 10 ng DNA
Synonym(e):
DNA Methylation Quantification, DNA methylation shift
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NACRES:
NA.54
UNSPSC Code:
41116158
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Unterstützung erhaltenGeneral description
Die Imprint™ methylierte DNA-Quantifizierungstechnologie bietet eine schnelle und verlässliche Methode zum Messen von globalen DNA-Methylierungsverschiebungen. Das Imprint methylierte DNA-Quantifizierungskit enthält alle Reagenzien, die zum Nachweis von relativen Mengen methylierter DNA erforderlich sind. Bis zu 200 ng aufgereinigte DNA werden an die Wells des Assay-Streifens gebunden. Die methylierte DNA wird durch die Einfang- und Nachweis-Antikörper nachgewiesen und anschließend kolorimetrisch quantifiziert. Die in der Probe vorhandene Menge von methylierter DNA ist proportional zu der gemessenen Absorption.
Application
Imprint® methyliertes DNA-Quantifizierungskit wird in der globalen DNA-Methylierungsanalyse eingesetzt.
Features and Benefits
- ELISA-basiertes Format
- Das Verfahren umfasst 4 leicht zu befolgende Schritte
- Alle Reagenzien werden bereitgestellt, einschließlich methylierter Kontroll-DNA
- Die Nachweisgrenze liegt bei 5 ng vollständig methylierter DNA
- Die Ausgangs-DNA kann so wenig wie 10–200 ng umfassen
- Das Verfahren ist innerhalb von 3,5 Stunden beendet
- Das 96-Well-Format ermöglicht die Option auf das Untersuchen einzelner Proben oder für Studien mit hohem Durchsatz
- Optimierte Antikörper und Reagenzien mit hoher Spezifizität für 5-mC; keine Kreuzreaktivität mit nicht methyliertem Cytosin
- Kann mit adhärenten und Suspensionszellen, Gewebe, Plasma und anderen Körperflüssigkeiten wie Speichel, Urin und Serum verwendet werden
Legal Information
Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany
Kit-Komponenten auch einzeln erhältlich
Produkt-Nr.
Beschreibung
SDB
- M8570Methylated Control DNA, 50 ng/μL
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Hazard Classifications
Aquatic Chronic 3 - Met. Corr. 1 - Skin Sens. 1
wgk
WGK 3
Lagerklasse
10 - Combustible liquids
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Felicitas Thol et al.
Journal of clinical oncology : official journal of the American Society of Clinical Oncology, 29(21), 2889-2896 (2011-06-15)
To study the incidence and prognostic impact of mutations in DNA methyltransferase 3A (DNMT3A) in patients with acute myeloid leukemia. A total of 489 patients with AML were examined for mutations in DNMT3A by direct sequencing. The prognostic impact of
Roman Thaler et al.
The British journal of nutrition, 101(5), 743-749 (2008-08-08)
The impact of nutrition on the epigenetic machinery has increasingly attracted interest. The aim of the present study was to demonstrate the effects of various diets on methylation and gene expression. The antioxidative enzyme mitochondrial superoxide dismutase (MnSOD) was chosen
Rauf Ahmad Najar et al.
The Journal of nutritional biochemistry, 53, 121-132 (2017-12-09)
The present study has been designed to determine the effect of folate modulation (deficiency/supplementation) with aging on the promoter methylation of tumor suppressor and proto-oncogenes to understand the underlying mechanism of epigenetic alterations. Folate deficiency was induced for 3 and
Maiko Suzuki et al.
Molecular medicine reports, 1(4), 465-471 (2008-07-01)
Vascular endothelial growth factor (VEGF) is a potent inducer of angiogenesis in oral squamous cell carcinoma (OSCC). In this study, we used the novel DNA methyltransferase inhibitor zebularine (Zeb) to investigate epigenetic influences on the secretion of VEGF-A in the
Global DNA methylation profile in medullary thyroid cancer patients
Ceolin L, et al.
Experimental and Molecular Pathology (2018)
Artikel
Global DNA Modification Kit (MDQ1) monitors DNA methylation via sandwich ELISA-like assay, aiding in epigenetic research.
Protokolle
Imprint ® Methylated DNA Quantification Kit (MDQ1) Protocol
Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.
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Could you please provide the sensitivity of the kit and explain what differences in methylation status it can detect?
1 Antwort-
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Are there any species restrictions for Product MDQ1, Imprint® Methylated DNA Quantification Kit?
1 Antwort-
The antibody works on 5-me-C, so any species with DNA that is methylated at the 5 position of cytidine residues will work.
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When using Product MDQ1, Imprint® Methylated DNA Quantification Kit, do I need to use all the wells in the plate at once?
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No, you can use as many wells as needed. You will want to ensure that all the wells that are not being used are covered well with Parafilm, Saran Wrap or some other plate sealer.
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When using Product MDQ1, Imprint® Methylated DNA Quantification Kit, can I dilute the entire antibody at once and save it for later?
1 Antwort-
No. A fresh dilution of the antibody should be prepared each time the kit is used. Diluting the antibody all at once will decrease its stability and it's effectiveness in the kit.
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How does the storage temperature relate to shipping conditions?
1 Antwort-
The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.
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What is the detection limit of Product MDQ1, Imprint® Methylated DNA Quantification Kit?
1 Antwort-
The detection limit of this kit is 5 nanograms of fully methylated DNA.
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Can I use chloroform to extract my DNA before using Product MDQ1, Imprint® Methylated DNA Quantification Kit?
1 Antwort-
Choroform extraction should not interfere with the protocol as long as the DNA was not eluted with the chloroform.
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What is the Department of Transportation shipping information for this product?
1 Antwort-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Is Product MDQ1, Imprint® Methylated DNA Quantification Kit, shipped in two parts?
1 Antwort-
Yes, Product MDQ1, Imprint® Methylated DNA Quantification Kit, is shipped in 2 parts. Part 1 contains, 2-8 °C components (W4267, D8568, C3493, D8818, D8693, S3447, and A4231) and Part 2 contains -20 °C components (M8570 and B6561).
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