107-15-3
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Product Information Sheet-11814770001
1,500 × g for 10 minutes at +2 to +8°C.
Remove buffer and add 3 ml Cellular Lysis Buffer per 1 × 107 cells (e.g. for 5 × 107 cells, add 15 ml Cellular
Lysis Buffer).
QCM™ Endothelial Cell Migration Assay
A+
La
t
FB
S+
La
t
BS
A+
CD
FB
S+
CD
0
1.0×107
2.0×107
3.0×107
4.0×107
5.0×107
6.0×107
7.0×107
8.0×107
9.0×107
1.0×108
1.1×108
1.2×108
1.3×108
1.4×108
BSA
FBS
BSA+Lat
FBS+Lat
The Effective Use of Protein Kinase Inhibitors
22 90 87 74 27
49 109 21 42 33 84 39 83 106 84 6 6 4 86 113 99 83 60 52 78 107 107
100 107 106 103 41 19 84 22 94 57 15
QCM™ Endothelial Cell Invasion Assay (24 well, fluorometric)
errors.
control 2% FBS CalceinAM only
0
5.0×106
1.0×107
1.5×107
2.0×107
2.5×107
3.0×107
3.5×107
Fl
uo
re
sc
en
ce
in
te
ns
ity
(c
ou
nt
s/
se
c)
Data Sheet - H107
Vol. 15, Abstr. No. 171.5 (1989).
CYCLODEXTRIN SOLUBILITY TABLE
Solubility in
Solubility in 45% w/v Solubility
COMPOUND (RBI Catalog Nos. in Parenthesis) Water
User Guide - UFC9010D
83 of 107
如何使用 Amicon® Ultra-15 离心过滤装置
1 . 向 Amicon® Ultra 过滤装置加入不超过 15 mL 的样品（如果使用定角转子则为 12 mL）。
说明：使用前检查离心器间隙。
2 . 将盖好盖子的过滤装置放入离心转子中，用一个类似的装置相互平衡。
3 . 当使用摆桶转子时, 以最大 4,000 × g 旋转约 15-40 分钟。
4
Product Information Sheet-11667327001
2.3 × 107 cells
1
2
3
4
5
http://sigmaaldrich.com
The Effective Use of Protein Kinase Inhibitors
22 90 87 74 27
49 109 21 42 33 84 39 83 106 84 6 6 4 86 113 99 83 60 52 78 107 107
100 107 106 103 41 19 84 22 94 57 15
User Guide - UFC8010D
Rev 10/21 84 of 107
性能
流速
影响流速的因素包括样品的浓度、起始体积、溶质的化学性质、相对离心力、
离心转子的角度、滤膜类型以及温度。图 1 和表 1 可用来估计达到给定的滤
出液体积或浓度所需的时间。4 mL 样品旋转时间一般约需 10 至 20 分钟。
尽管大部分样品在离心过程的前 10 至 15 分钟被过滤，但一般在旋转
15 至 20 分钟之后才能达到最低浓缩液体积
Product Information Sheet - MITOISO2
Press, Inc. (New York, NY) pp. 107-142 (1997).
2. Duan, S. et al., J. Biol. Chem., 278(2),
1346-1353 (2003).
3. Moss, D.W., and Bates, T.E., Eur. J. Neurosci.,
13(3), 529-538 (2001).
Product Information Sheet - 10411523001
11 626 353 001
NADH, approx. 100%, disodium salt 500 mg 10 107 735 001
NADPH, approx. 98%, tetrasodium salt 100 mg
500 mg
1 g
10 107 824 001
10 621 692 001
10 621 706 001
For life
Magna ChIRP¿ RNA Interactome Kits Analysis and Mapping of Chromatin: Noncoding RNA Interactions
Volume of Lysis Buffer ( µL)
T-75 75 ~0.5 x 107 500
T-225 225 ~1.3 x 107 1300
10 cm plate 78.5 ~0.5 x 107 500
15 cm plate 176.6 ~1.0
EZ- Magna ChIRP RNA Interactome Kit Analysis and Mapping of Chromatin: Noncoding RNA Interactions
Volume of Lysis Buffer ( µL)
T-75 75 ~0.5 x 107 500
T-225 225 ~1.3 x 107 1300
10 cm plate 78.5 ~0.5 x 107 500
15 cm plate 176.6 ~1.0
MIT0IS02 - Technical Bulletin
the cells.
4. Add 1–2.5 mL of the prepared 1×
Extraction Buffer A per 2–5 × 107 cells.
Incubate on ice for 10–15 minutes.
5. Homogenize the cells on ice using a
Dounce homogenizer, 10–30 strokes
Data Sheet - 102376
medium powder to the water.
3. Allow to dissolve by gentle stirring (300 rpm) for
15 minutes.
4. Add sodium bicarbonate (20 g/10 L) and stir until
dissolved (~15 minutes).
5. Measure the pH
Data Sheet - F3022
Motil. Cytoskel., 27, 108
(1994).
2. North, A., et al., J. Cell Sci., 107, 445 (1994).
3. North, A., et al., J. Cell Sci., 107, 437 (994).
4. Vandekerckhove, J., and Weber, K., Eur. J.
Biochem.
Microbiology - 100778 Cult-Dip combi - Instructions for use
Bacteria Count Agar (TTC-Agar)
Bacteria
per ml 103 104 105 106 107
11-109-5_PB_100778:08-111-1_PB_113682 03.01.12 10:23 Seite 3
Status: December 2011
Merck KGaA, 64271 Darmstadt
Process Guidance - Cellvento BHK-200
cells (WCB 3%), with 1×107 viable cells/mL in
Cellvento® BHK-200 medium + 3% serum + 10% DMSO
- Continue with step 5.
4
5th adaptation step from Cellvento® BHK-200
Data Sheet - A1978
Cytoskel., 27, 108-
116 (1994).
2. North, A.J., et al., J. Cell Sci., 107, 445-455 (1994).
3. North, A.J., et al., J. Cell Sci., 107, 437-444 (1994).
4. Vandekerckhove, J., and Weber, K., Eur. J.
Biochem
Product Information Sheet - CE0050
Protease Inhibitor Cocktail
before use.
2. Resuspend 106–107 cells in 600 µL of ice-cold
Lysis and Separation Working Solution.
3. Mix gently by pipetting up and down.
Vortex briefly.
Data Sheet - SAB4200257
monoclonal
Clone 107-1A4, purified from hybridoma cell culture
SAB4200257
Product Description
Monoclonal Anti-PSMA (mouse IgG1 isotype) is
derived from the hybridoma 107-1A4 produced by
Data Sheet - P4746
al. Neuroscience, 115, 425
(2002).
3. Ashcroft, F.M., et al., J Ion Channels and Disease
Ed 1, (2000).
4. Khakh, B.S., et al., Pharmacol Rev,. 53, 107
(2001).
5. Ding, Y., et al., J.
Product Information Sheet - GDI3
Procedure #1
Moderate
(3 to 100 mg tissue, 103-107 mammalian cells or 109 bacterial cells)
Procedure #2
Large (0.1 to 1.0 g tissue or 107-108 mammalian cells) Procedure #3
Other yeast cells Procedure
Product Information Sheet - A3854
Cell Motil. Cytoskel., 27, 108-
116 (1994).
2. North, A.J., et al., J. Cell Sci., 107, 445-455
(1994).
3. Sawyer, C., et al., Cancer Res., 63, 1667-1675
(2003).
4. Song, J., et al.
Product Information Sheet-rdlig-ro
. Efficiency of ligation >5 × 106 blunt end, >1 × 107 sticky
end.
Transformation without DNA, for example, with
1x DNA Dilution Buffer (Vial 3) alone.
Integrity of competent cells; no growth
Development of A Multiplex Immunoassay Panel for Simultaneous Quantification of Eight Gut Hormones Using the Luminex xMAP Technology
157, and 45 pg/mL, respectively. The spiking recoveries of these hormones in serum matrix
were 80-107% with reasonable intra-assay variation (<11%) and inter-assay variation
(<19%). This assay was also
Monoclonal Anti-Coilin antibody produced in mouse (C1862)
. Lab. Invest. Biol., 2,
129-221 (1903).
3. Hervas, J.P., et al., Am. J. Anat., 159, 164-170
(1980).
4. Bohmann, K., et al., J. Cell Sci., 19, 107-113
(1995).
Data Sheet - P9247
et al. Neuroscience, 115, 425
(2002).
3. Ashcroft, F.M., et al., Ion Channels and Disease Ed
1, (2000).
4. Khakh, B.S., et al., Pharmacol Rev,. 53, 107
(2001).
5. Ding, Y., et al., J.
Data Sheet - SAB4200173
2001).
3. Ali, B.R., et al., J. Cell Sci., 117, 6401-6412 (2004).
4. Zerial, M., and McBride, H., Nature Rev. Mol. Cell
Biol., 2, 107-117 (2001).
5. Lutcke, A., et al., J. Cell Sci., 107, 3437-3448
Data Sheet - SAB4200174
2001).
3. Ali, B.R., et al., J. Cell Sci., 117, 6401-6412 (2004).
4. Zerial, M., and McBride, H., Nature Rev. Mol. Cell
Biol., 2, 107-117 (2001).
5. Lutcke, A., et al., J. Cell Sci., 107, 3437-3448
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