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TRI Reagent® Protokoll
TRI Reagent ist ein einstufiges RNA-Isolierungsreagens, das die gleichzeitige Isolierung von DNA, RNA und Protein ermöglicht. In diesem Protokoll werden die Probenvorbereitung sowie die Extraktion verschiedener Proben und die Fehlerbehebung beschrieben.
Development of Organic Semiconductors from Highly Ordered Oligo and Polythiophenes
The soaring global demand for energy, coupled with the limited supply of fossil fuels, has increased the need for renewable, low-cost energy sources. Organic electronics have shown great promise for applications in lighting, power, and circuitry, with rapidly improving performance
Workflow for Preclinical Monoclonal Antibody Quantification
Pharmacokinetic (PK) kit enables a robust, high-throughput assay for quantification of human IgG1 antibody in animal sera by LC-MS/MS.
ISOGRO® Amplify Recombinant Protein Expression
Our company presents an informative page regarding the use of ISOTEC Stable Isotopes to Amplify Recombinant Protein Expression with ISOGRO.
Extraction of Nitroimidazoles from Milk and Eggs using Molecularly Imprinted Polymers
In this report, SupelMIP SPE – Nitroimidazoles was evaluated for the extraction of nitroimidazoles and metabolites from milk and egg.
Cultrex ® 3-D Culture Matrix™ Laminin I Protocol
3-D Culture Matrix™ Mouse Laminin I may be used as a gel or as a media additive to study cellular growth and differentiation in three dimensions in vitro.
The Endothelial Cell Transwell Migration and Invasion Assay
Cell culture protocol: the endothelial cell transwell migration and invasion assay used to study angiogenesis and cancer cell metastasis. Explore over 350 PromoCell products.
Glycosyltransferases
Glycosyltransferases were initially considered to be specific for a single glycosyl donor and acceptor, which led to the one enzyme-one linkage concept. Subsequent observations have refuted the theory of absolute enzymatic specificity by describing the transfer of analogs of some
Multiclass Pesticide Analysis of Soy Milk Using a Matrix-Compatible SPME Fiber
Pesticide analysis, SPME Fiber, supelco spme fiber, direct immersion spme, pesticide analysis methods, pesticide analysis by gc-ms, DI-SPME, overcoated SPME, overcoated fibers, soy milk analysis
A Comparison of Solid Phase Extraction (SPE) Products Used to Purify Peanut Paste Extract for Aflatoxin Analysis
Supel Tox AflaZea SPE produced exceptional analyte recoveries with good reproducibility for all aflatoxins while requiring less than half the sample volume required by competing products.HPLC analysis of all spiked samples after SPE cleanup showed that all compared SPE cartridges
How to Run an mPAGE™ Protein Gel Using a Bio-Rad Electrophoresis System
Instructions for setting up an mPAGE SDS-PAGE precast protein gel for gel electrophoresis using a Bio-Rad Mini-PROTEAN® electrophoresis system.
Periodic Table of the Elements
Download the Periodic Table of the Elements with names, atomic mass and number in printable .pdf and image format. Use the periodic table chart by element names in alphabetical order for quick research, reference and lab use.
Development of a New Advanced KSOM Mouse Embryo Media that Enables Dual-Use Embryo Culture and Cell Handling Applications
Advanced KSOM mouse embryo media that can be used as a single medium for both harvest and culture of mouse embryos to facilitate creation of transgenic knockout mice.
Utilizing ISOGRO® Supplementation of M9 Minimal Media to Enhance Recombinant Protein Expression
Utilizing ISOGRO® Supplementation of M9 Minimal Media to Enhance Recombinant Protein Expression.
Copper-Free Click Chemistry
Copper-free click chemistry is an alternative approach to click chemistry that proceeds at a lower activation barrier and is free of cytotoxic transition metal catalysts.
Analysis of Pesticide Residues in Cannabis Using QuEChERS Extraction and Cleanup Followed by GC/MS/MS Analysis
Compared to conventional PSA/C18/GCB cleanup, cannabis extracts cleaned with the Supel QuE Verde mixture showed lower GC/MS background, and better recoveries for many pesticides.
GenomePlex Whole Genome Amplification Kit Protocol (WGA1)
GenomePlex is a Whole Genome Amplification (WGA) method that allows the researcher to generate a representative, 500-fold amplification of genomic DNA
PDGFR
Sigma-Aldrich offers many products related to PDGFR for your research needs.
Mesoporous Silica and their Applications
Mesoporous Silica and their Applications
GenomePlex Whole Genome Amplification Kit Protocol (WGA1)
GenomePlex is a Whole Genome Amplification (WGA) method that allows the researcher to generate a representative, 500-fold amplification of genomic DNA
Alternative Energy Tutorial
A brief tutorial on alternative energy materials for advanced batteries and fuel cells, as well as high-purity inorganics, conducting polymers, and electrolytes.
Improving the Chromatographic Separation of DMB-Labeled Sialic Acids for the Comparison of Biosimilars to Reference Materials
A significantly improved HPLC-fluorescence method for DMB-NANA and -NGNA, and application of this method to compare 2 candidate biosimilar therapeutic proteins to their respective RMs.
Degrader Building Blocks for Targeted Protein Degradation
Protein Degrader Building Blocks are a collection of crosslinker-E3 ligand conjugates with a pendant functional group for covalent linkage to a target ligand.
Thermal Transitions of Homopolymers: Glass Transition & Melting Point
Literature values for the glass transition temperature, (Tg), and melting temperature, (Tm), are given for the more common homopolymers.
Pressure-Temperature Calculator for Solvents
Use the calculator to determine the boiling point or pressure of solvents based on the Antoine Equation and see solvent attributes. View a list of products based on the solvent CAS number.
Oncogenes and Tumor Suppressors Reprogram Metabolism
We present an article about how proliferating cells require the biosynthesis of structural components for biomass production and for genomic replication.
Sample Preparation for Size Exclusion Chromatography
Samples for chromatographic purification should be clear and free from particulate matter. Simple steps to clarify a sample before beginning purification will avoid clogging the column, can reduce the need for stringent washing procedures, and can extend the life of
Accelerated Osteocyte Differentiation of Human Mesenchymal Stem Cells in a Novel Xeno-free and Serum-free Osteogenesis Differentiation Media
Development of a novel serum-free and xeno-free human mesenchymal stem cell (MSC) osteocyte differentiation media.
Using ISOGRO® Supplementation of M9 Minimal Media to Enhance Recombinant Protein Expression
Stable isotope enrichment of recombinant proteins is necessary for most structure-function studies using NMR spectroscopy. The uniform incorporation of stable isotopes such as 13C, 15N and D into proteins is routinely accomplished by using bacterial expression systems such as Escherichia
SPME-GC-MS Determination of Phthalate Esters in Ramen Noodle Flavor Packets
A sensitive, quantitative, and reproducible SPME-GC/MS procedure was developed by Supelco for the extraction of phthalate esters from oily food matrices, such as the flavored oils included with ramen noodle kits.
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