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In Vitro Screening Method for Characterization of Macrophage Activation Responses.

Methods and protocols (2022-09-23)
Brandon W Lewis, Sonika Patial, Yogesh Saini
ZUSAMMENFASSUNG

Macrophage activation refers to the enhanced functionality of macrophages in response to endogenous or exogenous stimuli. Due to the existence of limitless stimuli and a multitude of receptors on macrophage surfaces, the nature of activation (or acquired functioning) can be specific to the encountering stimulus. This article describes a macrophage-activation screening platform in a 96-well format. The methodology involves the generation of bone marrow-derived macrophages, their activation into two extreme activation states, and screening of activated macrophages for expression of bonafide protein biomarkers. A high-throughput and stringent assay to determine macrophage activation markers developed in this article can be adapted for biomarker determination in pathological conditions and toxicant/drug safety screening.

MATERIALIEN
Produktnummer
Marke
Produktbeschreibung

Sigma-Aldrich
Rekombinantes Interferon-γ Protein der Maus, The Interferon-gamma protein (or IFN-gamma protein) is a regulatory protein produced by activated NK cells & CD4+TCRalpha/beta+, CD8+TCRalpha/beta+ & TCRgamma/delta+ T cells.