The number of tests depends on the specific assay protocol, the amount of DPPH used per test, and the concentration required. To determine the number of tests, refer to the assay protocol to calculate the amount of DPPH used per test and divide 1 gram by that amount.
D9132
2,2-Diphenyl-1-picrylhydrazyl
powder
Synonym(s):
1,1-Diphenyl-2-picrylhydrazyl radical, 2,2-Diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl, DPPH
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| Size/SKU | Availability | Price |
|---|---|---|
1 g | Check Cart for Availability | €128.00 |
5 g | Check Cart for Availability | €429.00 |
About This Item
Empirical Formula (Hill Notation):
C18H12N5O6
CAS Number:
Molecular Weight:
394.32
NACRES:
NA.22
PubChem Substance ID:
UNSPSC Code:
12352127
EC Number:
217-591-8
MDL number:
Beilstein/REAXYS Number:
1846081
Form:
powder
Product Name
2,2-Diphenyl-1-picrylhydrazyl,
form
powder
Quality Segment
color
green
mp
~135 °C (dec.) (lit.)
storage temp.
2-8°C
SMILES string
[O-][N+](=O)c1cc(c([N]N(c2ccccc2)c3ccccc3)c(c1)[N+]([O-])=O)[N+]([O-])=O
InChI
1S/C18H12N5O6/c24-21(25)15-11-16(22(26)27)18(17(12-15)23(28)29)19-20(13-7-3-1-4-8-13)14-9-5-2-6-10-14/h1-12H
InChI key
HHEAADYXPMHMCT-UHFFFAOYSA-N
General description
2,2-diphenyl-1-picrylhydrazyl is a free radical, which shows hydrogen acceptor ability towards antioxidants. Hence, it is commonly used in DPPH assay for measuring the antioxidant activity of different natural samples such as wine, fruits, herbal tea etc.
Application
- Stable p-radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) adsorbed at the elbows of 22×3 reconstructed Au (111): This study focuses on the single-molecule level investigation of the stable organic radical DPPH, exploring its adsorption and interaction characteristics on a gold surface, which is crucial for understanding metal-free magnetic phenomena at subnanometer scales (R Vranik et al., 2020).
Other Notes
Free radical
1 of 1
This Item | |||
|---|---|---|---|
| Quality Level 100 | Quality Level 100 | Quality Level 200 | Quality Level 100 |
| form powder | form solid | form - | form powder |
| mp ~135 °C (dec.) (lit.) | mp 171 °C (dec.) (lit.) | mp 197-200 °C (lit.) | mp 223-225 °C (lit.) |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. - | storage temp. - |
| color green | color - | color - | color - |
signalword
Danger
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Resp. Sens. 1 - Self-react. C - Skin Irrit. 2 - Skin Sens. 1
Storage Class
4.1A - Other explosive hazardous materials
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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2,2-Diphenyl-1-picrylhydrazyl (DDPH) Catalouge # D9132 1 gram can perform how many tests.
1 answer-
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How can I determine the shelf life / expiration / retest date of this product?
1 answer-
If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdfHelpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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How to use DPPH to detect quantity of antioxidant in cosmetic sample ? Do you have any method?
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This product is not tested for activity, however there are a number of publications available that focus on this assay. Please see the link below for an example: https://www.sciencedirect.com/science/article/pii/S1756464615000511
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What is the extinction coefficient of 2,2-Diphenyl-1-picrylhydrazyl, DPPH, Product D9132?
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The millimolar extinction coefficient (EmM) has been reported to be 19.07 in chloroform, measured at a wavelength of 332 nm. Reference: Organic Electronic Spectra Data (OESD), 1, p. 753 (1946-1952).
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What is the solubility of 2,2-Diphenyl-1-picrylhydrazyl, DPPH, Product D9132?
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We would expect this material to be soluble in chloroform and methanol. Quality control testing shows that a concentration of about 50mg/mL in chloroform can be achieved.
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At what wavelength is the absorbance maximum (lambda max) for 2,2-Diphenyl-1-picrylhydrazyl, DPPH, Product D9132?
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Using chloroform as the solvent, the lambda max is approximately 332 nm.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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