L040050

Cap A

configured for (ÄKTA^® and OligoPilot^®)

• NACRES: NA.21

Properties

• type: for DNA synthesis
• Quality Level: 200
• product line: Proligo Reagents
• storage condition: dry at room temperature
• impurities: ≤100 ppm water content (Karl Fischer)
• color: clear
• suitability: conforms to structure (GC, Formulation)
• compatibility: configured for (ÄKTA^® and OligoPilot^®)

Description

General description

Cap A solution contains acetic anhydride and tetrahydrofuran (THF).

Application

Cap A solution is used after the phosphoramidite reaction/coupling step. This solution aborts chains bearing unreacted 5′hydroxyl groups due to failure of reaction with activated phosphoramidite (typically, 1 to 2% of growing oligonucleotide chains in each synthesis cycle). These unreacted 5′hydroxyl groups are capped with an acetyl group and thus rendered unreactive for subsequent synthesis steps.

Preparation Note

Acetonitrile / N-Methylimidazole 8/2 (v/v)

Other Notes

Cap A solution, containing acetonitrile (ACN) and N-methylimidazole (NMI), is mixed in situ with Cap B1 and Cap B2 solution during the capping (acetylation) reaction. Pyridine is applied as a mild base while NMI provides a powerful acylation catalyst.

Legal Information

OligoPilot is a registered trademark of Cytiva

ÄKTA is a registered trademark of Cytiva

Safety Information

• Pictograms: GHS02,GHS05,GHS06
• Signal Word: Danger
• Hazard Statements: H225 - H302 + H332 - H311 - H314
• Precautionary Statements: P210 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 2 - Skin Corr. 1B
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 2
• Flash Point(F): 35.6 °F
• Flash Point(C): 2 °C