L040080

Cap A

configured for PerkinElmer, configured for Polygen

• NACRES: NA.21

Properties

• type: for DNA synthesis
• Quality Level: 200
• product line: Proligo Reagents
• storage condition: dry at room temperature
• concentration: 88.7-108.4 g/L (Acetic Anhydride content, titration)
• color: clear
• compatibility: configured for PerkinElmer; configured for Polygen

Description

General description

Cap A solution contains acetic anhydride and tetrahydrofuran (THF). It is used after the phosphoramidite reaction/coupling step.

Biochem/physiol Actions

Cap A solution aborts chains bearing unreacted 5′ hydroxyl groups due to failure of reaction with activated phosphoramidite (typically, 1 to 2% of growing oligonucleotide chains in each synthesis cycle). These unreacted 5′ hydroxyl groups are capped with an acetyl group and thus rendered unreactive for subsequent synthesis steps.

Preparation Note

Acetic Anhydride / Tetrahydrofurane 9.1/90.9 (v/v)

Safety Information

• Pictograms: GHS02,GHS05,GHS06,GHS08
• Signal Word: Danger
• Hazard Statements: H225 - H302 - H315 - H318 - H331 - H335 - H336 - H351
• Precautionary Statements: P210 - P280 - P301 + P312 - P304 + P340 + P311 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Carc. 2 - Eye Dam. 1 - Flam. Liq. 2 - Skin Irrit. 2
• Supplementary Hazards: EUH019
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 1
• Flash Point(F): -6.2 °F - closed cup
• Flash Point(C): -21.2 °C - closed cup