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574795

Sigma-Aldrich

TBE Buffer, 10X, Molecular Biology Grade

A 10X concentrate that can be diluted to a 1X solution containing 89 mM Tris, 89 mM boric acid, 2 mM EDTA, pH ~8.3.

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Synonym(s):
Tris-Borate-EDTA Buffer

grade

Molecular Biology

form

liquid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

foreign activity

DNase, RNase, protease, none detected

shipped in

ambient

storage temp.

15-25°C

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This Item
574797PPB009B6185
form

liquid

form

liquid

form

powder

form

solution

storage condition

OK to freeze

storage condition

OK to freeze, desiccated

storage condition

-

storage condition

-

foreign activity

DNase, RNase, protease, none detected

foreign activity

DNase, RNase, protease, none detected

foreign activity

DNAse, none detected, Nickase, none detected, Protease, none detected, RNAse, none detected

foreign activity

-

storage temp.

15-25°C

storage temp.

15-25°C

storage temp.

-

storage temp.

room temp

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

-

manufacturer/tradename

-

General description

A 10X concentrate that can be diluted to a 1X solution containing 89 mM Tris, 89 mM boric acid, 2 mM EDTA, pH ~8.3.
Tris, borate, and ethylenediaminetetraacetic acid (TBE) buffer is used to perform agarose gel electrophoresis of DNA and RNA. It consists of a weak acid that exists in neutral and anionic forms (e.g., COOH and COOspecies). Tris is a weak base, which occurs in either neutral or cationic forms (Tris-NH2 and Tris-NH3+). These ions maintain a low conductivity medium, transmit the electrical current, and buffer the pH. Ethylenediaminetetraacetic (EDTA) although not essential is added as a preventative since it chelates Mg2+ ions and inactivates potential DNA nucleases. TBE allows an effective resolution of DNA fragments and slightly improves the separation of smaller fragments. It also stabilizes nucleic acids against enzymatic degradation.

Application

TBE Buffer, 10X, Molecular Biology Grade-Calbiochem has been used as a component of polyacrylamide sequencing gels for DNA electrophoresis using amplified fragment length polymorphism (AFLP).

Warning

Toxicity: Irritant (B)

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Repr. 1B

Storage Class Code

6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

EU REACH SVHC Candidate List

CAS No.

EU REACH Annex XVII (Restriction List)

CAS No.

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Agarose gel electrophoresis of DNA and RNA is routinely performed using buffers containing either Tris, acetate, and EDTA (TAE) or Tris, borate, and EDTA (TBE). Gels are run at a low, constant voltage (∼10 V/cm) to minimize current and asymmetric

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