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Sigma-Aldrich

TAE Buffer, 10X, Molecular Biology Grade

A 10X concentrate that can be diluted to a 1X solution containing 40 mM Tris, 40 mM acetate, and 1 mM EDTA, pH ~8.3.

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Synonym(s):
Tris-Acetate-EDTA Buffer

form

liquid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated

technique(s)

electrophoresis: suitable

foreign activity

DNase, RNase, protease, none detected

shipped in

ambient

storage temp.

15-25°C

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This Item
57479357479585810
Sigma-Aldrich

Sigma-Aldrich

85810

STE buffer solution

Premium Grade
storage condition

OK to freeze, desiccated

storage condition

OK to freeze, desiccated

storage condition

OK to freeze

storage condition

-

technique(s)

electrophoresis: suitable

technique(s)

-

technique(s)

-

technique(s)

-

foreign activity

DNase, RNase, protease, none detected

foreign activity

DNase, RNase, protease, none detected

foreign activity

DNase, RNase, protease, none detected

foreign activity

-

storage temp.

15-25°C

storage temp.

15-25°C

storage temp.

15-25°C

storage temp.

-

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

-

General description

Tris, acetate, and ethylenediaminetetraacetic acid (TAE) buffer consists of tris(2-amino-2-[hydroxymethyl]-1,3-propanediol) base, acetate, and ethylenediaminetetraacetic acid (EDTA). This is one of the most used running buffers in nucleic acid electrophoresis. TAE buffer improves the separation or resolution of large DNA fragments.TAE, a standard buffer, is mainly used to perform agarose gel electrophoresis of DNA and RNA in slab gels. TAE is one of the commonly used buffers in every biochemistry and molecular biology laboratory.

Application

TAE Buffer, 10X, Molecular Biology Grade - Calbiochem has been used in 1X concentration for the preparation of agarose gel and as a running buffer in gel electrophoresis.

Warning

Toxicity: Irritant (B)

Preparation Note

Dilute 1:10 with H₂O to prepare a 1X solution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Agarose gel electrophoresis of DNA and RNA is routinely performed using buffers containing either Tris, acetate, and EDTA (TAE) or Tris, borate, and EDTA (TBE). Gels are run at a low, constant voltage (∼10 V/cm) to minimize current and asymmetric

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