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C8532

Sigma-Aldrich

Sodium citrate tribasic dihydrate

for molecular biology, ≥99%

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Synonym(s):
Citric acid trisodium salt dihydrate, Trisodium citrate dihydrate
Linear Formula:
HOC(COONa)(CH2COONa)2 · 2H2O
CAS Number:
Molecular Weight:
294.10
Beilstein:
6104939
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

grade

for molecular biology

Quality Level

Assay

≥99%

form

powder

pH

7.5-9 (25 °C, 29.4 g/L)

mp

>300 °C (lit.)

solubility

water: soluble 100 mg/mL

foreign activity

DNAse, none detected
Endonuclease, none detected
Exonuclease, none detected
NICKase, none detected
Protease, none detected
RNAse, none detected

SMILES string

O.O.[Na+].[Na+].[Na+].OC(CC([O-])=O)(CC([O-])=O)C([O-])=O

InChI

1S/C6H8O7.3Na.2H2O/c7-3(8)1-6(13,5(11)12)2-4(9)10;;;;;/h13H,1-2H2,(H,7,8)(H,9,10)(H,11,12);;;;2*1H2/q;3*+1;;/p-3

InChI key

NLJMYIDDQXHKNR-UHFFFAOYSA-K

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General description

Sodium citrate tribasic dihydrate is a stabilizer that imparts colloidal stability to gold nanoparticles (AuNPs).

Application

Sodium citrate tribasic dihydrate has been used:
  • to perform antibody retrieval for the immunohistochemical analysis of ovine ovarian sections
  • as a component of induction medium to culture Agrobacterium strain C58C1
  • to isolate human neutrophils from whole blood

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Protocols

Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.

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