12 - Non Combustible Liquids
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No. The 20% solution of Ficoll has a density of approximately 1.066 to 1.068 g/mL. Typically products such as Histopaque 1077-1 or Ficoll Paque have a density of approximately 1.076 to 1.078 g/mL. Such ready to use solutions are also osmotically balanced by the addition of a lower molecular weight chemical. Properly balanced solutions will typically have an osmolality in the range of approximately 280-300 mOsm/Kg. If a ready made product is required for the separation of mononuclear cells, products Histopaque 1077-1 or H8889 should be utilized.
No claims are made for sterility for F5415. If a sterile product is required for cell culture use, product F8636 has been gamma irradiated and is considered a sterile product. Product F8636 is a powder and does not come as a sterile solution.
Ficoll is a registered trademark. The trademark was formerly held by Pharmacia, but today Amersham Biosciences/GE Healthcare hold the trademark. Since Sigma Aldrich uses polysucrose type 400 in the preparation of the various Histopaque products, the name polysucrose 400 is utilized.
This is not recommended. Ficoll has a molecular weight in the range of 400,000. Even 20% solutions of such high molecular weight products have extremely low osmolality. Without the addition of other lower molecular weight chemical, solutions of 20%+ of Ficoll are considered extremely hypotonic. Cells exposed to hypotonic solutions would not maintain viability and are prone to bursting.
No. However there is a Product Information Sheet for product F4375. This product information sheet gives general information regarding Ficoll and possible uses. If mononuclear cells are to be separated, a better choice of products is Histopaque 1077-1, H8889 or one of the prefilled Accuspin tubes.
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Step-by-step hematopoietic stem cell culture protocols for isolation, expansion and differentiation of CD34+ hematopoietic progenitor cells including CFU assays. It can be cultured under defined conditions designed either to promote self-renewal and increase the number of primitive cells.