Merck
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D7058

Sigma-Aldrich

DirectLoad™ Wide Range DNA Marker

ready-to-use marker for DNA electrophoresis

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Synonym(s):
DirectLoad marker, agarose gel electrophoresis ladder, RTU DNA ladder, agarose gel electrophoresis marker, gel marker, RTU DNA marker, gel ladder, wide range ladder for gel electrophoresis, wide range marker for gel electrophoresis, wide range ladder
NACRES:
NA.25

Quality Level

100

form

liquid

usage

100 uses

suitability

suitable for electrophoresis (DNA)

storage temp.

−20°C

Related Categories

General description

Sigma′s DirectLoad Wide Range DNA Marker contains 16 fragments from 50 – 10,000 bp, with bands regularly spaced. Ten ul of the ladder can be loaded directly in a single lane on an agarose or polyacrylamide gel.

Application

Suitable for size determination of PCR-generated DNA fragments with either agarose or polyacrylamide gels.

Features and Benefits

  • Ready-to-load
  • Easy-to-use
  • Popular band sizes

Components

Sigma′s DirectLoad™ Wide Range DNA Marker is provided in a gel-loading solution of 2.5% Ficoll (Type 400), 0.0125% bromophenol blue, and 0.00625% xylene cyanol.

Other Notes

Contains 16 fragments, 50–10,000 bp which produce logically spaced bands on an agarose gel.
For optimal resolution, the recommended agarose gel concentration is 0.75 – 2.0%.

Legal Information

DirectLoad is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

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Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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