Merck
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F1418

Sigma-Aldrich

Ficoll® 400

BioXtra, Type 400-DL, lyophilized powder

Synonym(s):
Polysucrose 400
CAS Number:
MDL number:
NACRES:
NA.25

Quality Level

type

Type 400-DL

product line

BioXtra

form

lyophilized powder

impurities

≤0.0005% Phosphorus (P)
≤0.1% Insoluble matter

ign. residue

<0.1%

anion traces

chloride (Cl-): ≤0.05%
sulfate (SO42-): ≤0.05%

cation traces

Al: ≤0.0005%
Ca: ≤0.001%
Cu: ≤0.0005%
Fe: ≤0.0005%
K: ≤0.005%
Mg: ≤0.001%
NH4+: ≤0.05%
Na: ≤0.01%
Pb: ≤0.001%
Zn: ≤0.0005%

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Application

Ficoll® 400 is used together with sodium diatrizoate (Paque) to create Ficoll-Paque, a density mixture, used for the separation of cells and organelles, especially lymphocytes and monocytes from blood. Ficoll 400® may be used for the development of new cell and organelle separation systems. Ficoll 400 is used in tissue engineering and macromolecular conformation research for the development, evaluation and use of macromolecular crowding (MMC) systems and configurations.
Used for cell separation and organelle isolation.

Packaging

25, 100 g in poly bottle

Other Notes

A nonionic synthetic polymer of sucrose.

Legal Information

Ficoll is a registered trademark of Cytiva

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Soluble form of the endothelial adhesion molecule CD146 binds preferentially CD16+ monocytes.
Garibaldi S, Barisione C, Ghigliotti G, et al.
Molecular Biology Reports, 39(6), 6745-6752 (2011)
T Min et al.
Transplantation proceedings, 42(5), 1825-1829 (2010-07-14)
Sufficient and favorable biological functions of islets are major problems hindering xenotransplantation. The aim of the present study was to evaluate the effects on harvesting, purity, viability, and function of using improved Visipaque (iodixanol) and Ficoll-400 for adult porcine islet
Fahad M Almajid
Indian journal of pathology & microbiology, 54(4), 775-781 (2012-01-12)
Seronegative brucellosis is occasionally encountered in clinical practice especially in localized disease where diagnosis is reached mainly through positive blood culture. Cellular immune responses are pivotal for protection against intracellular bacteria such as Brucella. This study was performed to evaluate
Selective Expansion of CD34+ Cells from Mouse Bone Marrow Cultured on LH/P MP-Coated Plates with Adequate Cytokines.
Kishimoto S, Ishihara M, Kanatani Y, et al.
Journal of Tissue Engineering, 2, doi: 10-doi: 10 (2011)
Yanyan Xu et al.
Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery, 24(4), 406-409 (2010-05-13)
The purity and activity of islets will greatly affect the outcome of xenotransplantation therapy of type 1 diabetes mellitus. To set up an improved method of the isolation and purification of rat islets, which can obtain high-purity, high-yield, and high-viability

Protocols

Ficoll® 400 for Gradient Centrifugation

Ficoll 400® is a highly branched polymer formed by the copolymerization of sucrose and epichlorohydrin. Ficoll 400® is completely non-ionic. Because of the abundance of hydroxyl groups, Ficoll 400® is very hydrophilic and extremely water-soluble.

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