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Ficoll® 400

lyophilized powder, γ-irradiated, BioXtra, suitable for cell culture

Polysucrose 400
CAS Number:
MDL number:

Quality Level



product line



lyophilized powder


cell culture | mammalian: suitable

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Ficoll is a hydrophilic neutral highly branched polysaccharide use to establish density gradients to separate blood component cells and organelles. It is part of the separation medium called Ficoll-Paque.
Used for cell separation and organelle isolation.

Other Notes

A nonionic synthetic polymer of sucrose.

Legal Information

Ficoll is a registered trademark of Cytiva

Storage Class Code

11 - Combustible Solids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

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A Schoug et al.
Journal of applied microbiology, 108(3), 1032-1040 (2009-09-09)
To investigate the effect of freeze-dried Lactobacillus coryniformis Si3 on storage stability by adding polymers to sucrose-based formulations and to examine the relationship between amorphous matrix stability and cell viability. The resistance to moisture-induced sucrose crystallization and effects on the
Yann Peytour et al.
Transfusion, 50(10), 2152-2157 (2010-05-12)
Human CD34+ cells are mandatory to study many aspects of human hematopoiesis. Their low frequency in blood or marrow and ethical reasons limit their obtainment in large quantities. Leukoreduction filters (LRFs) are discarded after preparation of red blood cells. The
Naoaki Sakata et al.
Transplantation, 87(6), 825-830 (2009-03-21)
There is a recent focus on embolization of the portal vein by transplanted islets as a major cause of early graft loss. The resultant ischemia causes necrosis or apoptosis of cells within the liver. Thus, noninvasive assessment of the liver
Louise A Knight et al.
BMC cancer, 9, 38-38 (2009-01-30)
Previous data suggest that lipophilic statins such as fluvastatin and N-bisphosphonates such as zoledronic acid, both inhibitors of the mevalonate metabolic pathway, have anti-cancer effects in vitro and in patients. We have examined the effect of fluvastatin alone and in
Weihua Fei et al.
PLoS genetics, 7(7), e1002201-e1002201 (2011-08-11)
Lipid droplets (LDs) are important cellular organelles that govern the storage and turnover of lipids. Little is known about how the size of LDs is controlled, although LDs of diverse sizes have been observed in different tissues and under different


Ficoll® 400 for Gradient Centrifugation

Ficoll 400® is a highly branched polymer formed by the copolymerization of sucrose and epichlorohydrin. Ficoll 400® is completely non-ionic. Because of the abundance of hydroxyl groups, Ficoll 400® is very hydrophilic and extremely water-soluble.

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