Merck
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F9378

Sigma-Aldrich

Ficoll® 400

Type 400-DL, lyophilized powder

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Synonym(s):
Polysucrose 400
CAS Number:
26873-85-8
MDL number:
MFCD00081599

Quality Level

200

biological source

sugar beets (Beta vulgaris)

type

Type 400-DL

form

lyophilized powder

solubility

H2O: soluble 100 mg/mL, clear to slightly hazy, colorless to faintly yellow

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Application

Ficoll® 400 is used together with sodium diatrizoate (Paque) to create Ficoll-Paque, a density mixture, used for the separation of cells and organelles, especially blood derived lymphocytes and monocytes. Ficoll 400 may be used for the development of new cell and organelle separation systems. Ficoll 400 is used in tissue engineering and macromolecular conformation research for the development, evaluation and use of macromolecular crowding (MMC) systems and configurations.
Used for cell separation and organelle isolation.

Packaging

5, 10, 25, 100, 500 g in poly bottle

Other Notes

Dialyzed
A nonionic synthetic polymer of sucrose.

Legal Information

Ficoll is a registered trademark of Cytiva

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Bo Chen et al.
Tissue engineering. Part C, Methods, 19(8), 586-595 (2013-02-21)
Macromolecular crowding (MMC) has been shown to have a beneficial effect on production and maturation of extracellular matrices in a monolayer cell culture model. To explore its potential in tissue engineering, a mixture of Ficoll 70 and Ficoll 400 is
Lucie Kosinova et al.
Islets, 11(6), 129-140 (2019-09-10)
Instant Blood-Mediated Inflammatory Reaction (IBMIR) is a major cause of graft loss during pancreatic islet transplantation, leading to a low efficiency of this treatment method and significantly limiting its broader clinical use. Within the procedure, transplanted islets obstruct intrahepatic portal
Yanyan Xu et al.
Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery, 24(4), 406-409 (2010-05-13)
The purity and activity of islets will greatly affect the outcome of xenotransplantation therapy of type 1 diabetes mellitus. To set up an improved method of the isolation and purification of rat islets, which can obtain high-purity, high-yield, and high-viability
Mirna González-González et al.
Biotechnology progress, 30(3), 700-707 (2014-01-23)
Aqueous two-phase systems (ATPS) represent a promising strategy for the recovery of CD133(+) stem cells. This particular type of stem cells has great potential for research and clinical applications. Traditional [polyethylene glycol (PEG), dextran (DEX), and ficoll] and novel (Ucon)
Frank Brand et al.
Acta neuropathologica, 139(1), 175-192 (2019-09-02)
In search of novel genes associated with glioma pathogenesis, we have previously shown frequent deletions of the KIAA1797/FOCAD gene in malignant gliomas, and a tumor suppressor function of the encoded focadhesin impacting proliferation and migration of glioma cells in vitro
View All Related Papers

Protocols

Ficoll® 400 for Gradient Centrifugation

Ficoll 400® is a highly branched polymer formed by the copolymerization of sucrose and epichlorohydrin. Ficoll 400® is completely non-ionic. Because of the abundance of hydroxyl groups, Ficoll 400® is very hydrophilic and extremely water-soluble.

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