The protein content and enzymatic activity are determined on a lot-to-lot basis. Each value is reported in the lot-specific Certificate of Analysis. The minimum protein content is 50% and the minimum enzymatic activity is 10 units per milligram protein. A review of recent lots indicates typical protein concentrations of >60% and activities of 15 - 30 units per milligram protein. Please see the link below to review a sample or lot-specific Certificate:
https://www.sigmaaldrich.com/product/sigma/g5003#product-documentation
G5003
α-Glucosidase from Saccharomyces cerevisiae
Type I, lyophilized powder, ≥10 units/mg protein (using p-nitrophenyl α-D-glucoside as substrate.)
Synonym(s):
α-D-Glucosidase, α-D-Glucoside glucohydrolase, Maltase from yeast
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 100 units | Estimated to ship on21 July 2026fromSchnelldorf | €118.00 | |
| 1000 units | Estimated to ship on21 July 2026fromSchnelldorf | €431.00 |
About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-604-7
MDL number:
Specific activity:
≥10 units/mg protein (using p-nitrophenyl α-D-glucoside as substrate.)
type
Type I
Quality Level
form
lyophilized powder
specific activity
≥10 units/mg protein (using p-nitrophenyl α-D-glucoside as substrate.)
contains
phosphate buffer salts and EDTA as balance
composition
Protein, ≥50%
greener alternative product characteristics
Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
foreign activity
β-Glucosidase, α−galactosidase and β−galactosidase ≤0.1%
greener alternative category
, Enabling
relevant disease(s)
diabetes (management of non-insulin-dependent diabetes mellitus)
storage temp.
−20°C
Gene Information
bakers yeast ... MAL12(853209), MAL32(852602)
General description
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch hydrolysis research. For more information see the article in biofiles.
Application
α-glucosidase is used for the determination of α-amylase and the synthesis of various 1′-O-sucrose and 1-O-fructose esters. It was also used in the measurement of glycosidase inhibition.[1]
For the determination of α-amylase and the synthesis of various 1′-O-sucrose and 1-O-fructose esters
Biochem/physiol Actions
α-glucosidase hydrolyzes carbohydrates by acting on 1,4-α linkages. Inhibition of α-glucosidase is a prominent target in the management of non-insulin-dependent diabetes mellitus[2].
Hydrolysis of terminal, non-reducing 1→4-linked D-glucose residues with release of D-glucose.
Packaging
Sold on basis of p-nitrophenyl α-D-glucoside units.
Analysis Note
Protein determined by biuret.
Other Notes
One unit will liberate 1.0 μmole of D-glucose from p-nitrophenyl α-D-glucoside per min at pH 6.8 at 37 °C.
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
1 of 1
This Item | |||
|---|---|---|---|
| Gene Information bakers yeast ... MAL12(853209), MAL32(852602) | Gene Information bakers yeast ... MAL12(853209), MAL32(852602) | Gene Information - | Gene Information - |
| specific activity ≥10 units/mg protein (using p-nitrophenyl α-D-glucoside as substrate.) | specific activity ≥100 units/mg protein | specific activity ≥50 units/mg protein | specific activity 30-60 units/mg protein (biuret) |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
| UniProt accession no. | UniProt accession no. | UniProt accession no. - | UniProt accession no. - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Related Content
QC Methods
M Bianchi
Anatomischer Anzeiger, 169(5), 351-366 (1989-01-01)
In 13 horses from both sexes, between 5 months and 18 years of age, in good nutritional state, statistical evaluations of fat cell sizes were performed in 16 body regions. From direct and indirect measurements referred to the cell diameter
Matusorn Wongon et al.
Heliyon, 6(3), e03458-e03458 (2020-03-11)
Long-term diabetic complications are exacerbated by post-prandial hyperglycemia which could be ameliorated by α-glucosidase inhibitor including oxyresveratrol. Puag-Haad is an aqueous extract from Artocarpus lakoocha Roxb. containing ~65% oxyresveratrol. Oxyresveratrol is an inhibitor of isolated yeast α-glucosidase enzyme but has
Ivan Best et al.
Heliyon, 6(10), e05209-e05209 (2020-10-23)
Mauritia flexuosa L.f. is a palm tree which presents great morphological variability (morphotypes), represented mainly by the mesocarp color of its fruits. The objective of the study was to characterize the physicochemical and antioxidant properties of three morphotypes of Mauritia
Global Trade Item Number
| SKU | GTIN |
|---|---|
| G5003-1KU | 04061835545216 |
| G5003-100UN | 04061835556991 |
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what is its specific unit
1 answer-
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WHAT WOULD BE THE SUBSTRATE CONCENTRATION OF 4-Nitrophenyl α-D-glucopyranoside FOR 1 U/ml OF THE ENZYME?
1 answer-
Literature indicates that the substrate concentration for using 4-Nitrophenyl α-D-glucopyranoside with this enzyme is typically in the saturation range of 1 mM or higher.
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What is the molecular mass of the protein ? More information about the chain structure, number of sulfide bonds, or conditions required for its stability (ions)?
1 answer-
The molecular weight of this enzyme has not been determined. The chain structure, number of sulfide bonds, and other parameters have not been characterized. However, a similar recombinant version, product G0660, lists a molecular weight of approximately 68.5 kDa. Other sources report the molecular weight of a-Glucosidase from Saccharomyces cerevisiae at approximately 63 kDa.
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What is the best method to store a solution of this enzym? Which solvent and wat which temperature? How long can you store it without losing enzymatic activity?
1 answer-
Stock solutions of this enzyme may be aliquoted and stored at -20°C. It is recommended to use within 3 to 6 months.
Helpful?
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