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Merck

H8889

Sigma-Aldrich

Histopaque®-1077 Hybri-Max

liquid, sterile-filtered, BioReagent, suitable for hybridoma

Synonym(s):

Histopaque 1077

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€90.60
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€316.00

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100 ML
€90.60
500 ML
€316.00

About This Item

UNSPSC Code:
12352201
NACRES:
NA.75

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€90.60


In StockDetails


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sterility

sterile-filtered

Quality Level

product line

BioReagent

form

liquid

technique(s)

cell culture | hybridoma: suitable
single cell analysis: suitable

impurities

endotoxin, tested

density

1.077 at 25 °C (relative density)(lit.)

storage temp.

2-8°C

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This Item
1077110831A6929
density

1.077 at 25 °C (relative density)(lit.)

density

1.077

density

1.083

density

1.077 g/mL

form

liquid

form

liquid

form

liquid

form

solution

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

aseptically filled

technique(s)

cell culture | hybridoma: suitable, single cell analysis: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

Quality Level

400

Quality Level

400

Quality Level

400

Quality Level

400

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

General description

Histopaque®-1077 is a solution comprising polysucrose and sodium diatrizoate, adjusted to a density of 1.077 +/- 0.001 g/cm3.[1] This medium facilitates rapid recovery of viable lymphocytes from small volumes of whole blood. This technique is suitable for use in cell-mediated lympholysis and human lymphocyte antigen (HLA)2 typing. It may also be employed as the initial isolation step before the enumeration of T-, B-, and null lymphocytes. It is used to create a density medium for the purification of lymphocytes and other mononuclear cells.

Application

Histopaque®-1077 Hybri-Max has been used to isolate:
  • peripheral blood mononuclear cells (PBMCs) from heparinized blood by gradient centrifugation[2]
  • immune cells (lymphocytes) from peripheral blood[3]
  • PBMCs from blood[4]

Legal Information

Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany
Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Questions

1–2 of 2 Questions  
  1. Is there a product in your inventory that is comparable to the Corning product 25-072-CV?

    1 answer
    1. Upon comparison of the formulation and product description, the comparable product from MilliporeSigma is identified as product number H8889. Both products, 25-072 and H8889, have a density of 1.077 g/ml and share the same formulation components, including Diatrizoic acid at 96.219 and Polysucrose 400 at 61.363.

      Helpful?

  2. What is the formulation for H8889 Histopaque-1077 Hybri-Max?

    1 answer
    1. The formulation for H8889 Histopaque-1077 Hybri-Max consists of 5.7% polysucrose 400 and 9% sodium diatrizoate.

      Helpful?

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