Merck
All Photos(4)

P4630

Sigma-Aldrich

Protease from bovine pancreas

Type I, ≥5 units/mg solid

CAS Number:
EC Number:
MDL number:
eCl@ss:
32160410
NACRES:
NA.54

Quality Level

biological source

bovine pancreas

type

Type I

form

powder

specific activity

≥5 units/mg solid

storage temp.

−20°C

Related Categories

Application

Protease is an enzyme used to break down proteins by hydrolyzing peptide bonds. Protease is used to degrade proteins, to study protease inhibitors and to study thermal inactivation kinetics. Product P4630 is from bovine pancreas. Protease from bovine pancrease (type I) has been used for the extraction of hemicellulose.

Packaging

250 mg in poly bottle
1, 5, 10 g in poly bottle

Biochem/physiol Actions

Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate.

Quality

Crude

Unit Definition

One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent), unless otherwise indicated.

Pictograms

Exclamation markHealth hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Technical Information

Quotes and Ordering

P A Hedin et al.
The Journal of nutrition, 124(12), 2458-2465 (1994-12-01)
The southwestern corn borer, Diatraea grandiosella Dyar, is a major pest of corn, Zea mays L., in the southern United States. To search for plant allelochemicals, chemically defined insect diets are commonly used, but southwestern corn borer larvae did not
Werend Boesmans et al.
Glia, 67(6), 1167-1178 (2019-02-08)
Coordination of gastrointestinal function relies on joint efforts of enteric neurons and glia, whose crosstalk is vital for the integration of their activity. To investigate the signaling mechanisms and to delineate the spatial aspects of enteric neuron-to-glia communication within enteric
Sajna Anand Sadanandan et al.
Virus research, 223, 20-27 (2016-06-23)
Nora virus is an enteric virus that causes persistent, non-pathological infection in Drosophila melanogaster. It replicates in the fly gut and is transmitted via the fecal-oral route. Nora virus has a single-stranded positive-sense RNA genome, which is translated in four
U Klöckner
Pflugers Archiv : European journal of physiology, 424(3-4), 231-237 (1993-08-01)
Calcium-activated chloride currents were studied by the patch-clamp technique in vascular smooth muscle cells (VSMC) isolated from human mesenteric arteries. Bath application of 20 mM caffeine caused the cell membrane to depolarize by a calcium-activated inward current that peaked to
Marc Machnik et al.
Drug testing and analysis, 9(9), 1372-1384 (2016-09-24)
Methylxanthines positives in competition samples have challenged doping control laboratories and racing jurisdictions since methylxanthines are naturally occurring prohibited substances and often constituents of feed. For theobromine, an international threshold (renamed in International Residue Limit, IRL) of 2 µg/mL in urine

Protocols

Universal Protease Activity Assay using Casein as a Substrate

Measure and compare the activity of proteases with this non-specific protease activity assay using casein. Use this assay as a standardized procedure to determine the activity of proteases for quality control.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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