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Merck

P8811

Sigma-Aldrich

Protease from Streptomyces griseus

powder, BioReagent, suitable for mouse embryo cell culture, ≥3.5 units/mg solid

Synonym(s):

Actinase E, Pronase E

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100 MG
€52.90
1 G
€278.00
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€1,050.00

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100 MG
€52.90
1 G
€278.00
5 G
€1,050.00

About This Item

CAS Number:
EC Number:
EC Number:
MDL number:
UNSPSC Code:
12352207
eCl@ss:
32160410
NACRES:
NA.75

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€52.90


In StockDetails


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product line

BioReagent

Quality Level

form

powder

specific activity

≥3.5 units/mg solid

technique(s)

cell culture | embryo: suitable

impurities

starch, essentially free

storage temp.

−20°C

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1 of 4

This Item
P5147P6911537088
technique(s)

cell culture | embryo: suitable

technique(s)

-

technique(s)

-

technique(s)

-

form

powder

form

powder

form

powder

form

lyophilized

specific activity

≥3.5 units/mg solid

specific activity

≥3.5 units/mg solid

specific activity

-

specific activity

≥70,000 proteolytic units/g dry wt

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

impurities

starch, essentially free

impurities

-

impurities

-

impurities

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

-

Specificity

A mixture of at least three proteolytic activities including an extracellular serine protease. In general, serine proteases display a wide range of substrate specificities, which are believed to be mediated by an active site composed of one Asp, one His, and a Ser residue in the molecule. This enzyme prefers to hydrolyze peptide bonds on the carboxyl side of glutamic or aspartic acid.

Application

Protease is typically used in nucleic acid isolation procedures in incubations of 0.5-3.0 hours supplemented with 0.2% sodium dodecyl sulfate and 10 mM EDTA.
This enzyme is more active at a higher pH range than the known alkaline protease, showing the proteolytic activity even in 0.2N NaOH solution. This enzyme is useful for proteolysis of insoluble protein and for structure investigation of protein.

Other Notes

An unusually non-specific protease.

Quality

Contains approximately 25% calcium acetate.

Physical properties

Completely inactivated by heating above 80 °C for 15-20 minutes.

Unit Definition

One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).

Preparation Note

Collected from culture broth of S. griseus.

Pictograms

Health hazardExclamation mark

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Questions

1–2 of 2 Questions  
  1. What is the recommended reconstitution time for this product?

    1 answer
    1. This protease is solubilized at 37 °C in a concentration of 0.2 mg/mL in 10 mM sodium acetate/5 mM calcium acetate buffer, pH 7.5. The solution remains stable when refrigerated for 24 hours. There is no specified time for solubility.

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  2. What is the recommended expirtation date for P8811-100mg Lot #SLCB5965 rec'd 1/11/21?

    1 answer
    1. This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services

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