T111000

DMT-dT Phosphoramidite

• Synonym(s): 5′-O-(4,4′-dimethoxytrityl)-2′-deoxythymidine-3′-O-[O-(2-cyanoethyl)-N,N′-diisopropylphosphoramidite], 5′-O-[bis(4-methoxyphenyl)phenylmethyl]-2′-deoxythymidine, 3′-[2-cyanoethyl N,N-bis(1-methylethyl)phosphoramidite], DMT-dT amidite, Thymidine, 5′-O-[bis(4-methoxyphenyl)phenylmethyl]-, 3′-[2-cyanoethyl N,N-bis(1-methylethyl)phosphoramidite]
• Empirical Formula (Hill Notation): C₄₀H₄₉N₄O₈P
• CAS Number: 98796-51-1
• Molecular Weight: 744.81
• MDL number: MFCD00055063
• UNSPSC Code: 41116107
• PubChem Substance ID: 329826641
• NACRES: NA.51

Properties

• biological source: non-animal source
• Quality Level: 200
• type: for DNA synthesis
• product line: Proligo Reagents
• Assay: ≥99% (³¹P-NMR); ≥99.0% (reversed phase HPLC)
• form: powder or granules
• technique(s): oligo synthesis: suitable
• impurities: ≤0.3 wt. % water content (Karl Fischer)
• color: white to off-white
• solubility: water: soluble 0.00051 g/L at 20 °C
• λ: conforms (UV/VIS Identity)
• nucleoside profile: base: deoxythymidine; base protecting group: none; 2' protecting group: none; 5' protecting group: DMT; deprotection: fast/standard
• storage temp.: 2-8°C
• SMILES string: COc1ccc(cc1)C(OC[C@H]2O[C@H](C[C@@H]2OP(OCCC#N)N(C(C)C)C(C)C)N3C=C(C)C(=O)NC3=O)(c4ccccc4)c5ccc(OC)cc5
• InChI: 1S/C40H49N4O8P/c1-27(2)44(28(3)4)53(50-23-11-22-41)52-35-24-37(43-25-29(5)38(45)42-39(43)46)51-36(35)26-49-40(30-12-9-8-10-13-30,31-14-18-33(47-6)19-15-31)32-16-20-34(48-7)21-17-32/h8-10,12-21,25,27-28,35-37H,11,23-24,26H2,1-7H3,(H,42,45,46)/t35-,36+,37+,53?/m0/s1
• InChI key: UNOTXUFIWPRZJX-CEXSRUIHSA-N

Description

General description

DMT-dT Phosphoramidite belongs to the DNA Phosphoramidites group. Some key features of standard protected DNA phosphoramidite are mentioned below:

• Exocyclic amine functions are protected by a benzoyl group (dA(bz) anddC(bz)) or isobutyryl group (dG(ib))
• Recommended cleavage and deprotection conditions are 8 hours at 55 °Cor 24 hours at room temperature using concentrated ammonia solution, for standard base-protected oligonucleotides
• The high coupling efficiency of Proligo′s DNA phosphoramidites leads to high-yield and high-quality oligonucleotides