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T1895

Sigma-Aldrich

Thymidine

powder, BioReagent, suitable for cell culture

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Synonym(s):
1-(2-Deoxy-β-D-ribofuranosyl)-5-methyluracil, 1-(2-Deoxy-β-D-ribofuranosyl)thymine, 2′-Deoxythymidine, Thymine deoxyriboside, dT
Empirical Formula (Hill Notation):
C10H14N2O5
CAS Number:
Molecular Weight:
242.23
Beilstein:
89285
EC Number:
MDL number:
UNSPSC Code:
12352207
PubChem Substance ID:
NACRES:
NA.75

biological source

synthetic (organic)

Quality Level

product line

BioReagent

Assay

≥99%

form

powder

technique(s)

cell culture | mammalian: suitable

mp

186-188 °C (lit.)

solubility

water: 50 mg/mL, clear, colorless

SMILES string

CC1=CN([C@H]2C[C@H](O)[C@@H](CO)O2)C(=O)NC1=O

InChI

1S/C10H14N2O5/c1-5-3-12(10(16)11-9(5)15)8-2-6(14)7(4-13)17-8/h3,6-8,13-14H,2,4H2,1H3,(H,11,15,16)/t6-,7+,8+/m0/s1

InChI key

IQFYYKKMVGJFEH-XLPZGREQSA-N

Gene Information

human ... DTYMK(1841)

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General description

Thymidine is also referred to as pyrimidine deoxynucleoside. Deoxythymidine is a nucleoside present in DNA. In a DNA double stranded structure, thymidine pairs with deoxyadeninosine.

Application

Thymidine can be used in HT and HAT cocktails for hybridoma fusion, selection and cloning. It has been used:
  • as a nucleotide source in minimal essential medium to evaluate the outgrowth of the iSCNT-derived blastocysts
  • as a supplement in Dulbecco′s Modified Eagle Medium (DMEM) selection medium used for the production of anti-neurogranin (NRGN) antibodies
  • to prepare THMG (thymidine, hypoxanthine, glycine, methotrexate) and THG stock solution for cleansing mouse lymphoma cells

Biochem/physiol Actions

Thymidine is useful in cell synchronization during S-phase. In the salvage pathway of pyrimidines, pyrimidine phosphorylase reversibly converts thymine to thymidine.

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Human Biochemistry and Disease, 571-571 (2008)
Optimization in Drug Discovery: In Vitro Methods (2016)
Molecular Biology, Principles of Genome Function: Biology, Molecular biology (2016)
Dominique Desbouis et al.
Journal of medicinal chemistry, 51(21), 6689-6698 (2008-10-08)
Human cytosolic thymidine kinase (hTK1) has proven to be a suitable target for noninvasive imaging of cancer cell proliferation using radiolabeled substrates such as [ (18)F]fluorothymidine ([ (18)F]FLT). However, a thymidine tracer useful for single photon emission tomography (SPECT) based
Serum neurogranin measurement as a biomarker of acute traumatic brain injury
Yang J, et al.
Clinical Biochemistry, 43(13-14), 843-848 (2015)

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