U211200

DMT-2′Fluoro-dU Phosphoramidite

• Synonym(s): DMT-2′-Fluoro-dU amidite
• Empirical Formula (Hill Notation): C₃₉H₄₆FN₄O₈P
• CAS Number: 146954-75-8
• Molecular Weight: 748.78
• MDL number: MFCD11113919
• PubChem Substance ID: 329827613
• NACRES: NA.51

Properties

• biological source: non-animal source (no BSE/TSE risk)
• Quality Level: 200
• product line: Proligo Reagents
• Assay: ≥99% (³¹P-NMR); ≥99.0% (reversed phase HPLC)
• form: powder
• technique(s): oligo synthesis: suitable
• impurities: ≤0.3% water content (Karl Fischer); ≤0.5% P(III) Impurities 100-169ppm (³¹P-NMR); ≤0.5% single Impurity (reversed phase HPLC); ≤3% residual Solvent content
• color: white to off-white
• λ: conforms (UV/VIS Identity)
• suitability: conforms to structure for H-NMR; conforms to structure for LC-MS
• nucleoside profile: base: deoxyuridine; base protecting group: none; 2' protecting group: fluoro; 5' protecting group: DMT; deprotection: fast/standard
• storage temp.: −20°C
• SMILES string: COc1ccc(cc1)C(OC[C@H]2O[C@H]([C@H](F)[C@@H]2OP(OCCC#N)N(C(C)C)C(C)C)N3C=CC(=O)NC3=O)(c4ccccc4)c5ccc(OC)cc5
• InChI: 1S/C39H46FN4O8P/c1-26(2)44(27(3)4)53(50-24-10-22-41)52-36-33(51-37(35(36)40)43-23-21-34(45)42-38(43)46)25-49-39(28-11-8-7-9-12-28,29-13-17-31(47-5)18-14-29)30-15-19-32(48-6)20-16-30/h7-9,11-21,23,26-27,33,35-37H,10,24-25H2,1-6H3,(H,42,45,46)/t33-,35-,36-,37-,53?/m1/s1
• InChI key: HQHQPAYRJJMYQX-DJTPZYMWSA-N

Description

General description

DMT-2′Fluoro-dU Phosphoramidite is a 2′Fluoro phosphoramidite that is used to synthesize oligonucleotides that are more thermally stable and provide increased nuclease resistance.

Its key features are-

• Can be employed together with DNA or RNA phosphoramidites
• Recommended deprotection conditions are 8 hours at 55 °C using concentrated ammonia solution, or with AMA for 10 minutes at 65 °C
• Synthesis of 2′Fluoro oligonucleotides is similar to standard DNA synthesis but requires an elongated coupling time (recommended is 3 minutes compared to 90 seconds for DNA monomers)
• Consistent lot-to-lot high purity and performance
• Manufactured under a certified ISO 9001 quality system

Application

DMT-2′Fluoro-dU Phosphoramidite is suitable for use in the synthesis of 2′-fluoro modified oligonucleotide sequences by employing automated solid-phase chemistry.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable