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505013

Supelco

Discovery® RP-AmideC16 HPLC Column

5 μm particle size, L × I.D. 15 cm × 4.6 mm

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eCl@ss:
32110501

Agency

suitable for USP L60

Quality Level

feature

endcapped

manufacturer/tradename

Discovery®

extent of labeling

11% Carbon loading

parameter

≤70 °C temp. range
400 bar pressure (5801 psi)

technique(s)

HPLC: suitable

L × I.D.

15 cm × 4.6 mm

surface area

200 m2/g

surface coverage

2.6 μmol/m2

impurities

<10 ppm metals

matrix

silica gel, high purity, spherical base material

matrix active group

amide, alkyl phase

particle size

5 μm

pore size

180 Å

operating pH range

2-8

application(s)

food and beverages

separation technique

reversed phase

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This Item
50500550506450501321
particle size

5 μm

particle size

5 μm

particle size

5 μm

particle size

5 μm

matrix

silica gel, high purity, spherical base material

matrix

silica gel, high purity, spherical base material

matrix

silica gel, high purity, spherical base material

matrix

silica gel, high purity, spherical base material

matrix active group

amide, alkyl phase

matrix active group

amide, alkyl phase

matrix active group

amide, alkyl phase

matrix active group

-

pore size

180 Å

pore size

180 Å

pore size

180 Å

pore size

180 Å

technique(s)

HPLC: suitable

technique(s)

HPLC: suitable

technique(s)

HPLC: suitable

technique(s)

HPLC: suitable

General description

Discovery® RP-AmideC16 HPLC Column consists of a stationary phase made up of high-purity C16-silica that includes a polar-embedded amide function attached to the silica surface with a propyl group.

Application

Discovery RP-AmideC16 HPLC Column may be used for the separation of:
  • Impurities in drugs such as closantel, levamisole and azaconazole during their determination by high performance liquid chromatography-diode array detection (HPLC-DAD).
  • Vitamin B6 compounds in food samples during their determination using LC coupled with fluorescence detection (FLD).
  • Vitamin C and other fat soluble vitamins in mature seeds of Lathyrus L. taxa during their identification using HPLC with ultraviolet (UV) detection.
  • Zidovudine in combination with other pharmaceuticals in intravenous fluid mixtures during the determination using HPLC combined with photodiode array (PDA) detector.

Discovery® RP-AmideC16 HPLC column may be used for the separation of:
  • Phenolic compounds, purine alkaloids and theanine in Camellia species by high performance liquid chromatography (HPLC) equipped with photodiode array detector (PDA).
  • Proprietary pharmaceutical compounds using HPLC combined with computer-assisted screening and Plackett−Burman experimental design.
  • Hydrophilic bases by LC with principal component analysis (PCA).
  • 2,4-Dinitrophenylhydrazone derivatives by HPLC equipped with ultraviolet (UV) spectrometric detection.

Features and Benefits

  • Excellent retention and resolution of polar compounds
  • Unique selectivity compared to C18
  • Excellent peak shape and efficiency
  • Less hydrophobic than C18 columns
  • Compatible with 100% aqueous mobile phases
   

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Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Customers Also Viewed

HPLC separations of zidovudine and selected pharmaceuticals using a hexadecylsilane amide column.
Caufield WV and Stewart JT
Chromatographia, 54(9-10), 561-568 (2001)
Strategy for developing and optimizing liquid chromatography methods in pharmaceutical development using computer-assisted screening and Plackett-Burman experimental design
Li W and Rasmussen HT
Journal of Chromatography A, 1016(2), 165-180 (2003)
Determination of vitamin B6 compounds in foods using liquid chromatography with post-column derivatization fluorescence detection.
Vinas P, et al.
Chromatographia, 59(5-6), 381-386 (2004)
An improved HPLC method for simultaneous determination of phenolic compounds, purine alkaloids and theanine in Camellia species
Peng L, et al.
J. Food Compos. Anal., 21(7), 559-563 (2008)
Isomerization of aldehyde-2, 4-dinitrophenylhydrazone derivatives and validation of high-performance liquid chromatographic analysis
Uchiyama S, et al.
Journal of Chromatography A, 996(1-2), 95-102 (2003)

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