Merck
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58934

Supelco

SUPELCOSIL Suplex pKb-100 HPLC Column

5 μm particle size, L × I.D. 25 cm × 4.6 mm

eCl@ss:
32110501

Agency

suitable for USP L68

Quality Level

feature

endcapped: no

manufacturer/tradename

SUPELCOSIL

extent of labeling

12.5% carbon loading

parameter

≤70 °C temp. range
400 bar pressure (5801 psi)

technique(s)

HPLC: suitable

L × I.D.

25 cm × 4.6 mm

surface coverage

surface coverage 3.4 μmol/m2

matrix

silica gel, spherical particle platform

matrix active group

amide, alkyl phase

particle size

5 μm

pore size

120 Å

surface area

170 m2/g

pH range

2-7.5

application(s)

food and beverages

separation technique

reversed phase

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General description

Suplex pKb-100 columns feature the same bonded phase functionality as SUPELCOSIL LC-ABZ columns. These specially deactivated columns differ in that Suplex pKb-100 is not endcapped, while SUPELCOSIL LC-ABZ is endcapped. The absence of the end-capping reagent results in better performance from Suplex pKb-100 for the strongest basic compounds, while LC-ABZ is preferred when the sample also contains acids and/or zwitterions.

Application

SUPELCOSIL Suplex pKb-100 HPLC Column was used in HPLC purification of prepurified fly ovaries. It was also used for HPLC analysis, done for the determination of retinoid.

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Legal Information

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC
Suplex is a trademark of Sigma-Aldrich Co. LLC

Certificate of Analysis

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Certificate of Origin

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