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Buccal DNA Extraction & WGA Amplification Protocol
This protocol provides a simple and convenient method to isolate, amplify, and purify genomic DNA from buccal swabs. Buccal swabs are a convenient method of acquiring a DNA sample.
Reverse Transcription Protocol
Method for reverse transcription of RNA into DNA. Uses a premixed reagent that contains reverse transcriptase, dNTPs, primers, RNase inhibitor and buffer. Fast generation of cDNA.
Standard PCR Protocol
Learn standard PCR protocol steps and review reagent lists or cycling parameters. This method for routine PCR amplification of DNA uses standard Taq DNA polymerase.
RNA Handling When Using the First Strand cDNA Synthesis Kit for RT-PCR (AMV)
First Strand cDNA Synthesis Kit for RT-PCR (AMV).
TeloTAGGG Telomerase PCR ELISA Protocol & Troubleshooting
The TeloTAGGG Telomerase PCR ELISA is used for sensitive qualitative detection of telomerase activity.
Multiplex Real-Time PCR
Multiplex qPCR employing probe-based chemistries is a demanding application that often requires extensive optimization and validation.
Amplification of DNA Using Jumpstart™ REDTaq® DNA Polymerase (D8187)
Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.
Reverse Transcription Protocol Using SYBR Green Dye Detection
Perform reverse transcription (RT) using a reverse transcriptase enzyme and dNTPs. Use total RNA or a gene-specific approach so that only the RNA of interest is converted to cDNA.
Method for PCR amplification of Bacterial DNA with low contamination using MTP™ Taq DNA Polymerase (D7442)
MTP™ Taq DNA Polymerase is a recombinant thermostable enzyme from Thermus aquaticus expressed in E. coli and purified using a proprietary process to minimize levels of contaminating DNA.
End Point PCR Protocol for Long and Accurate DNA Amplification
Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures.
Protocol for Conjugating NHS-Ester Modifications to Amino-Labeled Oligonucleotides
Protocol for conjugating NHS-ester modifications to an oligonucleotide with an amino label.
Amplification of Damaged DNA with Restorase DNA Polymerase (R1028)
Method for amplification of DNA from damaged DNA sources. Particularly useful for DNA extracted from old samples.
SEQR - SeqPlex RNA Amplification Kit Protocol
The SeqPlex RNA Amplification kit provides a method for amplification of total RNA or isolated mRNA prior to entry into the workflows of the commonly used deep sequencing platforms.
Extract-N-Amp™ FFPE Tissue PCR Protocol
Protocol for PCR amplification of formalin-fixed paraffin embedded animal tissues using the Extract-N-Amp Tissue PCR kit.
Long & Accurate PCR with RedAccuTaq®
REDAccuTaq LA protocol offers high-fidelity amplification of long PCR fragments with direct gel loading capability.
Digital PCR
Digital PCR enables absolute quantification and detection of minority sequences against similar majority sequences, such as somatic mutations.
Hot Start dNTP Protocol
Hot start dNTP protocol enhances specificity in PCR by blocking DNA polymerase nucleotide incorporation during PCR.
Animal Tissue DNA-Extraction & WGA Amplification Protocol
GenomePlex® Whole Genome Amplification efficiently extracts DNA from animal samples for genomic analysis.
Standard Reverse Transcription Protocol
Reverse transcription analyzes mRNA gene expression, facing challenges like transcript half-life differences and temporal patterns.
Kapa Biosystems Taq DNA Polymerase and PCR Kits
Robust Taq DNA polymerase reagents and PCR kits for efficient extraction, purification, and amplification of routine and challenging DNA template sequences.
Installation & Use of Milli-Q® SQ 2Series Water Purification Systems
View instructional videos and download User Manuals for Milli-Q® SQ 2Series lab water systems. You’ll be up and running in no time. Buy online systems, accessories, cartridges and services.
dNTP Hot Start PCR Protocol
Hot Start dNTPs block DNA polymerase until heat activation, enhancing PCR specificity.
Applications with REDTaq™
Reviews the applications and benefits for RedTaq, including standard RedTaq, Hot Start RedTaq and RedTaq for genomic DNA PCR.
DNase I Recombinant, RNase-free Protocol
DNase I from bovine pancreas is a glycoprotein of Mr 37000. A special procedure is used to remove RNases from the DNase preparation.
Expand™ Long Template PCR System Protocol Troubleshooting
This polymerase mixture produces a high yield of PCR product from genomic DNA.
Long and Accurate PCR Amplification of DNA (D8045)
Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures. AccuTaq LA.
Extract-N-Amp™ FFPE Tissue PCR Protocol
The following procedure is a modification of the Extract-N-Amp™ Tissue PCR protocol.
GenomePlex® Single Cell Whole Genome Amplification Kit (WGA4) - Protocol
GenomePlex® is a Whole Genome Amplification (WGA) method that allows the researcher to generate a representative amplification of genomic DNA
ABI3900 Synthesizer Columns with CUTAG CPG Frits
Next generation CPG Frit support for reliable, cost-effective oligo synthesis from Proligo®, for ABI 3900 and MerMade synthesizers
Plant DNA Extraction & WGA Amplification Protocol
GenomePlex® Whole Genome Amplification has been used to amplify genomic DNA from soybean, corn, tomato, purple coneflower, and ginseng.
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