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OligoArchitect™ Assay Design
Efficient qPCR relies on good assay design. Since the invention of PCR, many parameters have been identified as important for assay quality, such as estimates of oligo temperature characteristics, GC content and folding properties.
Oligonucleotide Quality Control by Mass Spectrometry
Mass Spectrometry is the technology of choice for analyzing oligonucleotide synthesis. It enables the most sensitive detection of low levels of by-products, which can affect performance.
Colony PCR
Colony PCR reagents and our colony PCR protocol using REDExtract-N-Amp™ PCR ReadyMix™ and JumpStart™ REDTaq® PCR ReadyMix™ reagents.
ThermaStop™-RT PCR Additive
PCR additive interacts with reverse transcriptase at low temperatures to reduce priming problems that lead to nonspecific bands.
Whole Transcriptome Amplification of RNA from Low Cell-Number Samples
The efficacy of amplification of small quantities of total RNA with the Complete Whole Transcriptome Amplification Kit (WTA2) was examined in this study.
Troubleshooting PCR and RT-PCR Amplification
This page shows PCR and RT-PCR amplification troubleshooting.
5'/3' RACE Kit, 2nd Generation Troubleshooting
In addition to the troubleshooting provided in the product manual, most probably the efficiency of the tailing reaction performed by Terminal Transferase could be impaired. This could occur due to several reasons (which will not only affect the control reaction
Oligonucleotide Quality Control & Quality Assurance
The foundation of Sigma's manufacturing process is a robust QMS, which drives compliance to ISO 9001:2008 certification.
Custom LAMP Primers in Partnership with New England Biolabs
Primers manufactured using proprietary purification adjustments to perform optimally in loop-mediated isothermal amplification assays.
Working with PCR
General Considerations; Factors to Consider in RT-PCR; Prevention of Carryover Contamination; Troubleshooting
KAPA Long Range PCR Kits FAQs
Review some of the most asked questions regarding the KAPA Long Range PCR system, which is a blend of Taq DNA polymerase and an engineered archaeal DNA polymerase with proofreading capability.
KAPA3G Plant PCR Kits FAQs
Amplify up to 5 kb fragments from purified plant DNA extracted using commercial kits or CTAB-based methods. Review some of the most asked questions regarding direct PCR from leaf disks, seeds and other plant tissues.
KAPA Express Extract Kit FAQs
Extract DNA from crude samples for PCR applications, including qPCR, using the KAPA Express Extract Kit. See frequently asked questions regarding sample type, optimization and storage.
Extract-N-Amp Reagent: Effective PCR from FTA® Blood Cards
Extract-N-Amp simplifies DNA extraction and amplification, enabling PCR directly from FTA blood cards, reducing variability.
PCR/qPCR/dPCR Assay Design
PCR workflow's variability influenced by sample source, reverse transcription, and assay design, impacting reproducibility.
Water for Nuclease-sensitive Molecular Biology Studies
Nuclease-free water (or PCR water) is needed in molecular biology to avoid sample degradation during PCR, sequencing, etc. Ultrafiltration provides a quicker, safer, and more sustainable alternative to DEPC treatment when preparing RNase-free and DNase-free water.
FastStart™ Taq DNA Polymerase Protocol & Troubleshooting
PCR success relies on enzyme, buffer choice, template purity, primer concentration, and nucleotide quality.
MIQE: Real-Time PCR Experiment Standards
MIQE-compliant qPCR services support primer design, protocol development, and data analysis for faster product development and publication.
Introduction & Historical Timelines
Explore PCR's history, from discovery to Nobel Prize. Discover real-time PCR (qPCR) and digital PCR developments.
Array CGH Analysis with Whole Genome Amplification
aCGH technology evolves for high-res chromosomal analysis, needing a large DNA input per sample, advancing genetic research.
PCR Troubleshooting Tips
PCR troubleshooting protocols require exploring all potential error sources to ensure reliable results, addressing each problem independently.
Extract-N-Amp™ Plant PCR Kits
Extract-N-Amp kits extract and amplify genomic DNA, optimized for plant tissue without inhibition concerns.
WTA2 Amplicon Modification for NGS
Transplex Whole Transcriptome Amplification (WTA2) precisely amplifies RNA maintaining transcript levels in test and reference samples.
Locked Nucleic Acid & Minor Groove Binder / Eclipse Dark Quencher
Explore Locked Nucleic Acid and MGB. These oligo modifications offer stability and specificity in molecular analysis, making them ideal for research and diagnostic applications.
Oligonucleotide Quantification
DNA can be easily quantitated in a UV spectrometer due to its highly conjugated nature.
Quantitative PCR and Digital PCR Detection Methods
Fluorescent dyes or probes are included in PCR mixes to monitor the change in NA amplicon concentration as the reaction proceeds.
FastStart™ Taq DNA Polymerase, 5 U/μL Protocol & Troubleshooting
The choice of the PCR enzyme in combination with an appropriate buffer can profoundly affect PCR outcome.
ThermaGenix Product Overview
How to stop nonspecific bands and off target primers in PCR.
Optimizing Crude Sample Plant PCR
Overcoming potent inhibitors and sample diversity in direct plant PCR.
Polymerase Chain Reaction (PCR) Basics
Polymerase chain reaction (PCR) is a technique that results in exponential amplification of a target DNA sequence.
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