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Protein mass spectrometry

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LC-UV-MS Method Development for Antibody Drug Conjugates Using a Non-Toxic ADC-Mimic
SigmaMab Antibody Drug Conjugate Mimic, is a non-toxic drug mimic utilized as a standard for mass spectrometry and high performance liquid chromatography.
Production of Dual-labelled Proteins
Refinement of protein dual-labeling protocol using EnPresso® B Defined Nitrogen-free showcased by Vernalis, Ltd. for drug discovery.
Mass Spectrometry of Glycans
Explore mass spectrometry analysis of glycans for glycomic & glycoproteomic neutral & acidic glycan analysis. See a general mass spec glycan analysis procedure.
Antibody Drug Conjugate Mimic Enables LC-MS Method Development Without Risk
Here we show how LC and MS methods may be optimized using a non-toxic surrogate of the ADC, an “ADC-mimic”, that behaves very similarly to the Cys-linked ADC Adcetris (Seattle Genetics).
Standard Methods 6040D: GC Analysis of Geosmin and 2-MIB on SLB®-5ms after SPME using 50/30 µm DVB/Carboxen/PDMS Fiber
Separation of 2-Isopropyl-3-methoxypyrazine 20 ppt; 2-Isobutyl-3-methoxypyrazine; 2-Methylisoborneol; (±)-Geosmin 10 ppt
Peptide Mapping LC-MS/MS Analysis Workflow for Adalimumab
An optimized LC-MS/MS based workflow for low artifact tryptic digestion and peptide mapping of monoclonal antibody, adalimumab (Humira) using filter assisted sample preparation (FASP).
Purification of Serum Peptides for MALDI Mass Spectrometry with Amicon® Ultra Centrifugal Filters
Biomarkers play an essential role in the drug discovery and development process.
Workflow and Analysis of Stressed and Unstressed Antibody Samples By Size Exclusion Chromatography
A complete SEC-UV workflow for quantifying monoclonal antibody fragments, monomers, and aggregates, including procedure for a system suitability test and heat stressing of antibodies.
High-Throughput Glycoprofiling Workflow for Rituximab
SEC-MS protocol for rapid glycoprofiling of monoclonal antibodies with antibody purification and mass spectrometer calibration.
Protocol for Purification, Optional Reduction, and SEC-MS Analysis of a Monoclonal Antibody
A complete workflow for the intact and middle-up mass analysis of reduced and non-reduced monoclonal antibodies based on SEC-MS with sample preparation by protein-A affinity clean-up.