HPLC Analysis of Acidic Drugs on Chromolith^® Performance 100- 2 mm

column

Chromolith^® Performance 100 x 2 mm (1.52006.0001)

mobile phase

[A] acetonitrile; [B] ammoniumacetate 20mM pH=6,8; (28:70, A:B)

flow rate

380 μL/min (u=2mm/s)

pressure

4619 psi (32 bar)

column temp.

ambient

detector

230 nm (cell: 1.4 μL)

injection

1.0 μL

sample

Indoprofen, Naprofen, Ethacrynic acid, Ibuprofen, Indomethacine