HPLC Analysis of Folic Acid Syrup on Chromolith^® High Resolution RP-18

sample preparation

Pipette out 15 ml of syrup in 50 ml volumetric flask. Add diluent to make up the volume.Sonicate for 30 min. Fiter through 0.2 μm filter paper.

column

Chromolith High Resolution RP-18 endcapped, 100 x 4.6 mm (1.52022.0001)

mobile phase

[A] 1.5 g of hexane 1-sulfonate in 1000 ml water containing 10 ml of glacial acetic acid; [B] acetonitrile;

flow rate

1.0 mL/min

pressure

1203 psi 83 Bar

column temp.

30 °C

detector

277 nm cell: 10 μL

injection

10 μL

sample

10 ppm in diluent (Diluent: Dissolve 6.8 g of KH2PO4 in 1000 ml water. Adjust pH to 6.8 with 10% NaOH solution)