324715

Lys-C Protease

Precise cleavage at lysine residues, suitable for mass spectrometry, from Lysobacter enzymogenes

• Synonym(s): Endoproteinase Lys-C, Sequencing Grade, Lysobacter enzymogenes
• CAS Number: 72561-05-8
• EC Number: 3.4.21.50 (BRENDA, IUBMB)
• MDL number: MFCD02253020
• UNSPSC Code: 12352202
• NACRES: NA.54

Properties

• Product Name: Endoproteinase Lys-C, Sequencing Grade, Lysobacter enzymogenes,
• grade: Proteomics Grade
• Quality Level: 100
• Assay: ≥90% (SDS-PAGE)
• form: lyophilized
• specific activity: ≥200 units/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated (hygroscopic)
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

Native endoproteinase Lys-C from Lysobacter enzymogenes. Serine protease that specifically hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine in peptides and proteins. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Inhibited by aprotinin, DFP, leupeptin, and TLCK. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.

Physical form

Lyophilized from 50 mM HEPES, 10 mM EDTA, 5 mg/ml raffinose, pH 8.0.

Preparation Note

Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 2 days at 4°C or for up to 1 month at -20°C.

Reconstitute in 50 µl of distilled H₂O.

Other Notes

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol Tos-Gly-Pro-Lys-pNA per min at 25°C, pH 7.7.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Harmful (C)