VP002
VirusExpress® 293 AAV Production Cells
Pharma Manufacturing
Synonym(s):
HEK293 cells for AAV production
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General description
VirusExpress 293 AAV Production Cells are derived from the HEK293 cell line (ATCC CRL-1573) through single cell cloning. The parental HEK293 cell line was established by transformation of human embryonic kidney cells with sheared human adenovirus 5 DNA (Graham F.L. et al. 1977). A 4-kb adenoviral genome fragment is known to have integrated into chromosome 19 and encodes for E1A/E1B proteins. Our clonal derivative eliminates variability from the population while also being adapted to suspension, serum-free conditions. Suspension culture and chemically defined medium allow scalability into stirred tank bioreactors for yields that can meet commercial needs.
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recommended
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Articles
Scaling up viral vector production using adherent cell culture systems is challenging. Learn how suspension cell culture systems benefit large-scale bioprocessing.
A step-by-step overview of suspension-based, transient transfection bioreactor process development and scaleup of lentivirus production.
To address scalability challenges of AAV manufacturing, we developed an HEK293 suspension cell line that can be used across many serotypes. Get the data in this article.
The current status and future manufacturing of AAV-based gene therapies is discussed in this podcast transcript, including how to streamline large-scale manufacturing.
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A transfection-based solution to viral vector production using a suspension cell line, chemically defined medium, and a process with proven performance at scale.
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