14385C

EX-CELL^® CD HEK293 Viral Vector Medium

Name: EX-CELL<SUP>®</SUP> CD HEK293 Viral Vector Medium
Brand: SIGMA

Chemically defined, animal component-free, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

EX-CELL® CD HEK293 Viral Vector Medium

Synonym(s):

HEK293/HEK293T medium for viral vector production

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About This Item

UNSPSC Code:

12352207

Key Documents

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sterility

sterile-filtered

product line

EX-CELL^®

form

liquid

technique(s)

cell culture | mammalian: suitable (suspension)

components

L-glutamine: no

shipped in

wet ice

storage temp.

2-8°C

Quality Level

500

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This Item	14380C	24381C	14366C
SAFC 14385C EX-CELL^® CD HEK293 Viral Vector Medium	SAFC 14380C EX-CELL^® CD Insect Cell Medium	24381C EX-CELL^® CD Insect Cell Medium	SAFC 14366C EX-CELL^® Advanced CHO Fed-batch Medium
sterility sterile-filtered	sterility sterile-filtered	sterility -	sterility sterile-filtered
technique(s) cell culture \| mammalian: suitable (suspension)	technique(s) cell culture \| insect: suitable	technique(s) cell culture \| insect: suitable	technique(s) cell culture \| mammalian: suitable (suspension)
form liquid	form liquid	form dry powder	form liquid
components L-glutamine: no	components NaHCO₃: no L-glutamine: yes	components L-glutamine: yes	components L-glutamine: no
product line EX-CELL^®	product line EX-CELL^®	product line EX-CELL^®	product line EX-CELL^®
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C

General description

EX-CELL CD HEK293 Viral Vector Medium is an animal origin-free, chemically defined cell culture medium for clinical and commercial manufacturing of viral vectors for gene and cell therapies. The medium is intended for processes implementing (polyethylenimine) PEI transfection reagents. EX-CELL CD HEK293 Viral Vector Medium is part of the VirusExpress^® Lentiviral Production Platform and the medium of choice for VirusExpress 293T Lentiviral Production Cells (VP001). The medium is also suitable as a standalone solution for other HEK293 and HEK293T cell lines for AAV and lentiviral manufacturing. EX-CELL CD HEK293 Viral Vector Medium has been formulated to support high suspension cell densities with minimal clumping, high transient transfection efficiency when used with PEI, and high viral titers in shake flask and stirred tank bioreactor formats. Viral vector production using this medium is a scalable, suspension process that does not require a media exchange or feed post-transfection.

EX-CELL CD HEK293 Viral Vector Medium has been designed with long-term needs for manufacturing in mind, with a sustainable raw material supply chain, custom packaging options, and careful material and manufacturing process selection. The product is produced in an ISO9001-certified facility following the principles of cGMP according to IPEC-PQG.

Application

EX-CELL CD HEK293 Viral Vector Medium was developed to support VirusExpress Production Platforms. It performs well within the platform as well as with other HEK293 clones. The following white paper demonstrates the use of one of the prototype media formulations in developing a scalable, transient transfection-based suspension process for lentiviral vector production using the VirusExpress 293T Lentiviral Production cells:

Legal Information

EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany

VirusExpress is a registered trademark of Merck KGaA, Darmstadt, Germany

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Description

Pricing

59202C

L-Glutamine Solution 200 mM, 29.23 mg/mL in saline, solution, suitable for cell culture

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

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Articles

Bioreactor Process Development and Scale-Up for Transient Transfection-Based Lentivirus Manufacturing in Suspension Cell Culture

A step-by-step overview of suspension-based, transient transfection bioreactor process development and scaleup of lentivirus production.

Scalability Challenges of Viral Vector Manufacturing

Scaling up viral vector production using adherent cell culture systems is challenging. Learn how suspension cell culture systems benefit large-scale bioprocessing.

High Performing Platforms for Lentivirus and AAV Manufacturing

Upstream gene therapy optimization maximizes viral vector titers; partner with technology experts for HEK293, HEK293T, and Sf9 cell solutions.

Protein Fingerprinting of a Viral Vector, AAV5

Method development for protein fingerprinting of AAV serotype 5 using both intact mass analysis and peptide mapping, to determine critical quality attributes for gene therapy, utilizing three different columns.

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EX-CELL^® CD HEK293 Viral Vector Medium