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71643

Sigma-Aldrich

di-Sodium hydrogen phosphate dihydrate

BioUltra, for molecular biology, ≥99.0% (T)

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Synonym(s):
sec-Sodium phosphate, Disodium hydrogen phosphate dihydrate, Disodium phosphate, Sodium phosphate dibasic dihydrate
Linear Formula:
Na2HPO4 · 2H2O
CAS Number:
Molecular Weight:
177.99
EC Number:
MDL number:
UNSPSC Code:
12161700
PubChem Substance ID:
NACRES:
NA.25

grade

for molecular biology

Quality Level

product line

BioUltra

Assay

≥99.0% (T)

form

crystals

impurities

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected
≤0.001% total nitrogen (N)

pH

8.9-9.2 (25 °C, 0.5 M in H2O)

solubility

H2O: 0.5 M at 20 °C, clear, colorless

anion traces

chloride (Cl-): ≤10 mg/kg
sulfate (SO42-): ≤50 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

λ

0.5 M in H2O

UV absorption

λ: 260 nm Amax: 0.03
λ: 280 nm Amax: 0.03

application(s)

diagnostic assay manufacturing

SMILES string

O.O.[Na+].[Na+].OP([O-])([O-])=O

InChI

1S/2Na.H3O4P.2H2O/c;;1-5(2,3)4;;/h;;(H3,1,2,3,4);2*1H2/q2*+1;;;/p-2

InChI key

KDQPSPMLNJTZAL-UHFFFAOYSA-L

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Application

Sodium phosphate dibasic dehydrate has been used in the preparation of antifreeze solution to analyse the transduction pattern in the rat central nervous system (CNS) and in the preparation of sodium phosphate buffer.

Other Notes

Gel electrophoresis - running buffer component for denaturing gels containing formamide

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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