Merck
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A2095

Millipore

Monoclonal Anti-HA−Agarose antibody produced in mouse

clone HA-7, purified immunoglobulin, PBS suspension

Synonym(s):
Monoclonal Anti-HA, Anti-HA, Anti-Influenza Hemagglutinin
MDL number:
NACRES:
NA.56

biological source

mouse

Quality Level

conjugate

agarose conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

HA-7, monoclonal

form

PBS suspension

isotype

IgG1

capacity

20-50 nmol/mL elution capacity (HA-tagged fusion protein)
30-50 nmol/mL binding capacity (HA-tagged fusion protein)

shipped in

wet ice

storage temp.

2-8°C

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General description

The HA tag is a 9-amino acid tag, with the sequence YPYDVPDYA (N-Tyr-Pro-Tyr-Asp-Val-Pro-Asp-Tyr-Ala-C), derived from the hemagglutinin (HA) protein at amino acid residues 98-106 in the HA sequence. The HA tag contains a high proportion of charged amino acid residues, which makes the HA tag likely to form a strong antibody recognition site. Monoclonal Anti-HA Agarose Conjugate recognizes native as well as denatured/reduced forms of HA-tagged proteins. This product is reactive with N-or C-terminal HA-tagged fusion proteins that are expressed in E. coli or in mammalian cells.

Specificity

The antibody recognizes native as well as denatured-reduced forms of HA-tagged proteins and is reactive with N- or C-terminal HA-tagged fusion proteins expressed in E. coli or in mammalian cells.

Immunogen

synthetic peptide corresponding to a fragment of human influenza virus hemagglutinin (HA) known as HA-tag, conjugated to KLH

Application

Suitable for use in immunoprecipitation assays and for immunoaffinity purification of HA-tagged fusion proteins.

Physical form

Suspension in 0.01 M phosphate buffered saline, containing 15 mM sodium azide

Preparation Note

Prepared using cyanogen bromide-activated agarose. Specific antibody concentration is 2.0 - 2.4 mg/mL settled resin.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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