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Ethylenediaminetetraacetic acid tetrasodium salt dihydrate

99.0-102.0% (titration)

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EDTA tetrasodium salt, Edathamil, Tetrasodium ethylenediaminetetraacetate dihydrate
Linear Formula:
CAS Number:
Molecular Weight:
EC Number:
MDL number:
PubChem Substance ID:

Quality Level


99.0-102.0% (titration)



reaction suitability

reagent type: chelator


H2O: soluble, clear, colorless

SMILES string




InChI key


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General description

Ethylenediaminetetraacetic acid (EDTA) is a polyprotic acid and a metal chelating agent. It comprises four carboxylic acid and two amine groups with lone-pair electrons that can chelate calcium and several other metal ions. 


Ethylenediaminetetraacetic acid tetrasodium salt dihydrate has been used:
  • in the decalcification of femurs for the histoprocessing and staining of osteochondral tissue
  • as a component of NH4Cl lysis buffer for erythrocyte lysis and bulk characterization
  • for the isolation of crypts from the connective tissues of mice

Biochem/physiol Actions

Ethylenediaminetetraacetic acid (EDTA) is used to eliminate enzyme inhibition by traces of heavy metals, and to inhibit enzymes that require divalent cations as cofactors.
Ethylenediaminetetraacetic acid (EDTA) is used to treat patients poisoned with heavy metal ions. It functions as a chelator of the zinc ion in the active site of metalloproteases. EDTA inhibits other metal ion-dependent proteases such as calcium-dependent cysteine proteases. It might interfere with biological events which are metal ion-dependent. EDTA inhibits platelet aggregation and is the most preferred anticoagulant for platelet counts. It associates with other active agents to modulate microorganisms and biofilms, like citric acid, alcohol, antibiotics, polyhexamethylene biguanide (PHMB) quaternary ammonium compounds, and other antiseptics. EDTA is used as an anticoagulant for hematological testing, as it preserves the cellular components and morphology of blood cells. 

Signal Word


Hazard Statements

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - STOT RE 2

Target Organs

Respiratory Tract

Storage Class Code

11 - Combustible Solids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Palaniappan Sethu et al.
Analytical chemistry, 76(21), 6247-6253 (2004-11-02)
Leukocyte isolation from whole blood to study inflammation requires the removal of contaminating erythrocytes. Leukocytes, however, are sensitive to prolonged exposure to hyper/hypoosmotic solutions, temperature changes, mechanical manipulation, and gradient centrifugation. Even though care is taken to minimize leukocyte activation
Giuseppe Banfi et al.
Clinical chemistry and laboratory medicine, 45(5), 565-576 (2007-05-09)
Anticoagulants are used to prevent clot formation both in vitro and in vivo. In the specific field of in vitro diagnostics, anticoagulants are commonly added to collection tubes either to maintain blood in the fluid state for hematological testing or
A Chevrier et al.
Osteoarthritis and cartilage, 15(3), 316-327 (2006-09-30)
Marrow-stimulation techniques are used by surgeons to repair cartilage lesions although consistent regeneration of hyaline cartilage is rare. We have shown previously that autologous blood can be mixed with a polymer solution containing chitosan in a glycerol phosphate (GP) buffer
Geoffroy Goujon et al.
Advanced healthcare materials, 10(11), e2100059-e2100059 (2021-04-24)
Oxidative stress, which is one of the main harmful mechanisms of pathologies including ischemic stroke, contributes to both neurons and endothelial cell damages, leading to vascular lesions. Although many antioxidants are tested in preclinical studies, no treatment is currently available
Michael L Salinas et al.
Biophysical journal, 118(4), 885-897 (2019-10-22)
The increasing prevalence of adult and adolescent obesity and its associated risk of colorectal cancer reinforces the urgent need to elucidate the underlying mechanisms contributing to the promotion of colon cancer in obese individuals. Adiponectin is an adipose tissue-derived adipokine


Cathepsin B is a lysosomal cysteine proteinase with broad specificity. This protocol uses Nα–CBZ–Arg–Arg–7–amido–4–methylcoumarin as the substrate for fluorometric detection of Cathepsin B activity.


Objective: To standardize a procedure for the recycling micro-assay of β-NAD and β-NADH

Enzymatic Assay of α-Glucosidase by the Modified Boehenger Procedure (EC

Enzymatic assay of lipase type XIII from Pseudomonas sp. using a coupled enzyme system of glycerol kinase and glycerophosphate oxidase (EC

This procedure may be used for all Ficin products.

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