Merck
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FLAGIPT1

Millipore

FLAG® Immunoprecipitation Kit

Synonym(s):
Anti-ddddk, Anti-dykddddk
NACRES:
NA.32

usage

 kit sufficient for 50 reactions

Quality Level

analyte chemical class(es)

proteins (FLAG® Affinity Gels)

technique(s)

immunoprecipitation (IP): suitable

shipped in

wet ice

storage temp.

−20°C

General description

Sigma′s FLAG Immunoprecipitation kit allows a rapid and efficient immunoprecipitation and elution of an active FLAG-tagged protein. The immunoprecipitation is performed with the ANTI-FLAG M2 affinity gel, which is a highly specific monoclonal antibody covalently attached to agarose resin. The use of affinity resin allows an efficient binding of FLAG-tagged proteins without the need for preliminary steps and calibrations. The immunoprecipitated FLAG-tagged proteins can be efficiently eluted from the resin with acidic conditions or by competition with FLAG peptide. The immunoprecipitated proteins can be detected for their size, post-translational modifications, and interactions on gel electrophoresis, and by activity assays.

Application

Browse additional application references in our FLAG® Literature portal.

Features and Benefits

  • Utilizes highly specific ANTI-FLAG M2 affinity gel
  • No preliminary steps or calibrations
  • 3X FLAG peptide provides efficient and gentle elution through direct competition

Other Notes

All prodedures to be performed at 2-8°C.

Legal Information

3xFLAG is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Kit Components Also Available Separately

Product No.
Description
SDS

  • A2220ANTI-FLAG M2 affinity gel 1 mL

  • SAE01943X FLAG peptide 1 mg

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Aquatic Chronic 3 - Eye Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

SDS

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