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HIS-Select® Elution Buffer


Quality Level

General description

Elution buffer is a major solvent in affinity chromatography. Elution buffer is used to wash away unbound proteins at first and at a greater concentration it releases the desired protein from the ligand. It is important that the elution buffer works quickly without changing the function or activity of the desired protein.


HIS-Select® Elution Buffer has been used in the elution of recombinant coagulase protein and tomoregulin 

Physical form

Contains 250 mM Imidazole, 0.3 M sodium chloride, 50 mM sodium phosphate, pH 8.0.

Legal Information

HIS-Select is a registered trademark of Sigma-Aldrich Co. LLC


Exclamation markHealth hazard

Signal Word


Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

EU REACH Annex XVII (Restriction List)


Certificate of Analysis

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Certificate of Origin

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