SHC016

MISSION^® pLKO.1-puro Non-Target shRNA Control Plasmid DNA

Name: MISSION<SUP>®</SUP> pLKO.1-puro Non-Target shRNA Control Plasmid DNA
Brand: SIGMA
Price: 3170 DKK

Targets no known genes from any species

Synonym(s):

MISSION^® Control Vectors, negative control, negative shRNA control, non-target control, non-target shRNA, non-target shRNA control, shRNA control

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About This Item

UNSPSC Code:

41106609

NACRES:

NA.51

MDL number:

MFCD07785395

Concentration:

500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)

Key Documents

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product line

MISSION^®

concentration

500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)

Quality Level

200

shipped in

dry ice

storage temp.

−20°C

Related Categories

Gene Editing & Functional Genomics

Genetic Screening

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This Item	SHC216	SHC001	SHC016H
Sigma-Aldrich SHC016 MISSION^® pLKO.1-puro Non-Target shRNA Control Plasmid DNA	Sigma-Aldrich SHC216 MISSION^® TRC2 pLKO.5-puro Non-Target shRNA Control Plasmid DNA	Sigma-Aldrich SHC001 MISSION^® pLKO.1-puro Empty Vector Control Plasmid DNA	Sigma-Aldrich SHC016H MISSION^® pLKO.1-puro Non-Target shRNA Control Transduction Particles, High Titer
concentration 500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)	concentration 500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)	concentration 500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)	concentration ≥1x10⁹ VP/ml (via p24 assay)
product line MISSION^®	product line MISSION^®	product line MISSION^®	product line MISSION^®
shipped in dry ice	shipped in dry ice	shipped in dry ice	shipped in dry ice
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −70°C
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200

General description

The MISSION pLKO.1-puro Non-Target shRNA Control Plasmid DNA is a lentivirus plasmid vector. The vector contains an shRNA insert that does not target any known genes from any species, making it useful as a negative control in experiments using the MISSION shRNA library clones. This allows one to examine the effect of transfection of a short-hairpin on gene expression and interpret the knockdown effect seen with shRNA clones. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The Non-Target shRNA Control Plasmid DNA is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.

Application

MISSION^® pLKO.1-puro non-target shRNA control plasmid DNA has been used as a control during transduction:

in tumor cells for multicolour imaging
in human adult low calcium temperature keratinocytes
in mouse embryonic fibroblasts, to study the biological functions of IP6K1 (inositol hexakisphosphate kinase)
to study the function of Zac1 (zinc finger protein regulating apoptosis and cell cycle arrest) expression in astroglial differentiation

To see more application data, protocols, vector maps visit sigma.com/shrna.

Legal Information

Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense.

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

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MISSION^® pLKO.1-puro-CMV-TagRFP^™ Positive Control Plasmid DNA, Contains a gene encoding TagRFP

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Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Deletion of inositol hexakisphosphate kinase 1 (IP6K1) reduces cell migration and invasion, conferring protection from aerodigestive tract carcinoma in mice

Jadav RS, et al.

Cellular Signalling, 28(8), 1124-1136 (2016)

Brain tumour cells interconnect to a functional and resistant network

Osswald M, et al.

Nature, 528(7580), 93-93 (2015)

Peroxiredoxin 2 nuclear levels are regulated by circadian clock synchronization in human keratinocytes

Avitabile D, et al.

The International Journal of Biochemistry & Cell Biology, 53(7580), 24-34 (2014)

Zac1 regulates astroglial differentiation of neural stem cells through Socs3

Schmidt EU, et al.

Stem Cells, 31(8), 1621-1632 (2013)

3D collagen architecture induces a conserved migratory and transcriptional response linked to vasculogenic mimicry.

D O Velez et al.

Nature communications, 8(1), 1651-1651 (2017-11-23)

The topographical organization of collagen within the tumor microenvironment has been implicated in modulating cancer cell migration and independently predicts progression to metastasis. Here, we show that collagen matrices with small pores and short fibers, but not Matrigel, trigger a

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MISSION® pLKO.1-puro Non-Target shRNA Control Plasmid DNA