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Merck

T9777

Trypsin-chymotrypsin inhibitor

lyophilized powder

Synonym(s):

Bowman-Birk Inhibitor

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About This Item

CAS Number:
37330-34-0
UNSPSC Code:
12352202
NACRES:
NA.77
MDL number:
MFCD00132532

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Product Name

Trypsin-chymotrypsin inhibitor from Glycine max (soybean), lyophilized powder

biological source

Glycine max (soybean)

form

lyophilized powder

composition

Protein, 70-90% biuret

storage temp.

2-8°C

Quality Level

200

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This Item
T2327T9003T9767
Trypsin-chymotrypsin inhibitor from Glycine max (soybean) lyophilized powder

Sigma-Aldrich

T9777

Trypsin-chymotrypsin inhibitor from Glycine max (soybean)

Trypsin Inhibitor from Glycine max (soybean) BioUltra, lyophilized powder, ≥95% (Kunitz inhibitor, SDS-PAGE)

Sigma-Aldrich

T2327

Trypsin Inhibitor from Glycine max (soybean)

Trypsin inhibitor from Glycine max (soybean) lyophilized powder

Sigma-Aldrich

T9003

Trypsin inhibitor from Glycine max (soybean)

Trypsin inhibitor from Glycine max (soybean) BioReagent, For use as a marker in SDS-PAGE

Sigma-Aldrich

T9767

Trypsin inhibitor from Glycine max (soybean)

biological source

Glycine max (soybean)

biological source

Glycine max (soybean)

biological source

Glycine max (soybean)

biological source

Glycine max (soybean)

Quality Level

200

Quality Level

200

Quality Level

300

Quality Level

200

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

powder

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

composition

Protein, 70-90% biuret

composition

-

composition

-

composition

-

Analysis Note

1 mg protein will inhibit ≥0.5 mg trypsin with activity of ~10,000 BAEE units per mg protein or ≥1.0 mg chymotrypsin with activity of ~40 BTEE units per mg protein.

Application

Bowman Birk protease inhibitor prevents radiation-induced carcinogenesis by a reduction of incorrect DNA repairs, resulting in a reduced amount of dicentric chromosomes.
Trypsin-chymotrypsin inhibitor from Glycine max (soybean) has been used for flow cytometric separase activity assay.[1] It has also been used to inhibit trypsin and chymotrypsin for in vitro gastrointestinal (GI) digestion studies.[2]

General description

Highly cross-linked with seven disulfide bridges, Bowman-Birk inhibitor has spatially distinct domains for trypsin and chymotrypsin inhibition.
Trypsin-chymotrypsin inhibitor belongs to the family of plant protease inhibitors. It is mainly found in the legume (Fabaceae) and cereal (Poaceae) families. Trypsin-chymotrypsin inhibitor consists of many internal disulfide bonds between conserved Cys residues, which maintains structural stability and active conformation of the inhibitory loops.[3] Trypsin-chymotrypsin has anticarcinogenic properties.[4]

Other Notes

One trypsin unit = ΔA253 of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C. Reaction volume = 3.2 mL (1 cm light path).
View more information on Trypsin Inhibitor.

Physical form

Lyophilized powder containing phosphate buffer salts, pH 7.6

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000490415
0000490417
0000490416
0000490416
0000490417

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Evidence for ancient origins of Bowman-Birk inhibitors from Selaginella moellendorffii
James AM, et al.
Plant Cell, 29(3), 461-473 (2017)
Alfonso Clemente et al.
Molecular nutrition & food research, 54(3), 396-405 (2009-11-04)
Bowman-Birk inhibitors (BBI) from soybean and related proteins are naturally occurring protease inhibitors with potential health-promoting properties within the gastrointestinal tract. In this work, we have investigated the effects of soybean BBI proteins on HT29 colon adenocarcinoma cells, compared with
Epitopes resistance to the simulated gastrointestinal digestion of beta-lactoglobulin submitted to two-step enzymatic modification
Villas-Boas M, et al.
Food Research International, 72, 191-197 (2015)
Yu Liu et al.
Viruses, 10(10) (2018-10-17)
The Bowman‒Birk inhibitor (BBI), a protease inhibitor derived from soybeans, has been extensively studied in anti-tumor and anti-inflammation research. We recently reported that BBI has an anti-HIV-1 property in primary human macrophages. Because HSV-2 infection plays a role in facilitating
Increased separase activity and occurrence of centrosome aberrations concur with transformation of MDS
Ruppenthal S, et al.
PLoS ONE, 13(1), e0191734-e0191734 (2018)
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Articles

Chymotrypsin

Analytical Enzyme Chymotrypsin: Chymotrypsin is produced in the acinar cells of the pancreas as the inactive precursor, chymotrypsinogen.

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