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Ascentis® Express Biphenyl Column

2.7 μm particle size, L × I.D. 10 cm × 2.1 mm

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stainless steel hardware

Quality Level


suitable for USP L11

product line







1 ea of


60 °C max. temp.
9000 psi max. pressure (600 bar)


HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

L × I.D.

10 cm × 2.1 mm

surface area

135 m2/g


<5 ppm metals


particle platform (Fused-Core)
superficially porous particle

matrix active group

biphenyl phase

particle size

2.7 μm

pore size

90 Å pore size

operating pH



food and beverages

separation technique

reversed phase

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General description

The Ascentis® Express biphenyl phase chemistry offers alternative selectivity for pharmaceutical analytes and drug metabolites that are not well retained or difficult to resolve on traditional alkyl (C18) and phenyl bonded phases. Ascentis Express HPLC columns, through the use of Fused-Core® particle technology, can provide you with both the high speed and high efficiencies of sub-2 ?m particles while maintaining lower backpressures. The combination of high efficiency and low backpressure benefits UHPLC users, as well as conventional HPLC users. Visit the Ascentis Express home page for more information on this new column technology.

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Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

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Determination of plasma protein binding using a SupelTM C18 pin device based BioSPME method for efficient sample preparation, along with a comparison to the equilibrium dialysis method.

The application note describes a comparative analysis of plasma protein binding results for free/bound drugs using two different sample extraction steps, with and without agitation.

Learn about Supel™ BioSPME C18 high-throughput devices to determine plasma protein binding and decrease the sample preparation time to less than an hour.

Comparative analysis of Supel™ BioSPME 96-Pin device with a rapid equilibrium dialysis technique for accuracy of measured values, sample cleanliness, and workflow time in drug protein binding.

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HPLC-UV analysis of active compounds in marijuana-infused edibles for potency testing; complex matrix requires precise methods.

Rapid potency testing of marijuana-infused edibles using LC/MS on a biphenyl stationary phase detected eleven cannabinoids.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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